Genetic variation and the fate of beneficial mutations in asexual populations.
Genetic variation and the fate of beneficial mutations in asexual populations.
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DOI：
10.1534/genetics.111.128942
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发表时间：
2011-07
期刊：
影响因子：
3.3
通讯作者：
Desai MM
中科院分区：
文献类型：
--
作者：
Lang GI;Botstein D;Desai MM
新出现的有益突变的命运取决于许多因素，例如种群规模以及在种群中积累的其他突变的可用性和适合性影响。事实证明，很难理解这些因素如何影响特定突变的轨迹，因为实验主要集中在表征从少数进化群体中出现的成功克隆。在这里，我们展示了一项大规模平行实验的结果，该实验旨在测量数百个实验酵母种群中新的有益突变的可能命运的全谱，无论这些突变最终成功与否。使用可以通过荧光检测到特定类别有益突变的菌株，我们在1000代人中跟踪了592个独立群体中这些有益突变的轨迹。我们发现，单个突变提供的适应优势所起的作用令人惊讶地小。相反，潜在的“背景”遗传变异在我们最初的克隆种群中迅速产生，并在决定进化种群中每个个体有益突变的命运中发挥关键作用。
The fate of a newly arising beneficial mutation depends on many factors, such as the population size and the availability and fitness effects of other mutations that accumulate in the population. It has proved difficult to understand how these factors influence the trajectories of particular mutations, since experiments have primarily focused on characterizing successful clones emerging from a small number of evolving populations. Here, we present the results of a massively parallel experiment designed to measure the full spectrum of possible fates of new beneficial mutations in hundreds of experimental yeast populations, whether these mutations are ultimately successful or not. Using strains in which a particular class of beneficial mutation is detectable by fluorescence, we followed the trajectories of these beneficial mutations across 592 independent populations for 1000 generations. We find that the fitness advantage provided by individual mutations plays a surprisingly small role. Rather, underlying “background” genetic variation is quickly generated in our initially clonal populations and plays a crucial role in determining the fate of each individual beneficial mutation in the evolving population.
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影响因子：
3.3
作者：
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通讯作者：
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影响因子：
56.9
作者：
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DOI：
10.1073/pnas.0705778104
发表时间：
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