Early exposure to intermediate-frequency magnetic fields alters brain biomarkers without histopathological changes in adult mice.
Early exposure to intermediate-frequency magnetic fields alters brain biomarkers without histopathological changes in adult mice.
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DOI：
10.3390/ijerph120404406
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发表时间：
2015-04-22
影响因子：
--
通讯作者：
Kunugita N
中科院分区：
文献类型：
--
作者：
Win-Shwe TT;Ohtani S;Ushiyama A;Kunugita N
最近，我们报道了中频磁场（IF-MF）暴露瞬时改变记忆功能相关基因的mRNA表达水平在成年雄性小鼠的海马。然而，IF-MF暴露在大脑发育过程中对神经生物标志物的影响尚未得到澄清。在本研究中，我们研究了发育期间IF-MF暴露对3周龄和7周龄雄性小鼠海马中神经和免疫标记物的影响。从器官形成期第7天至第17天，将妊娠C57 BL/6 J小鼠暴露于IF-MF（21 kHz，3.8 mT），每天1小时。在青春期，一些IF-MF暴露的小鼠被进一步分为暴露组、恢复组和假暴露组。暴露组在出生后第27天至48天再次暴露于IF-MF。采用实时荧光定量RT-PCR方法检测海马mRNA的表达，免疫组化分析检测小胶质细胞的活化。与对照组相比，IF-MF暴露小鼠中NR 1和NR 2B以及转录因子（CaMKIV，CREB 1），炎症介质（COX 2，IL-1 β，TNF-α）和氧化应激标志物血红素加氧酶（HO）-1的表达水平在7周龄小鼠中显著增加，但在3周龄小鼠中没有增加。小胶质细胞活化在对照组和其他组之间没有差异。这项研究提供了第一个证据表明，早期暴露于IF-MF可逆地影响NMDA受体，其相关的信号通路，和炎症介质在年轻的成年小鼠海马;这些变化是短暂的，并在暴露终止后恢复，没有组织病理学变化。
Recently we have reported that intermediate-frequency magnetic field (IF-MF) exposure transiently altered the mRNA expression levels of memory function-related genes in the hippocampi of adult male mice. However, the effects of IF-MF exposure during brain development on neurological biomarkers have not yet been clarified. In the present study, we investigated the effect of IF-MF exposure during development on neurological and immunological markers in the mouse hippocampus in 3- and 7-week-old male mice. Pregnant C57BL/6J mice were exposed to IF-MF (21 kHz, 3.8 mT) for one hour per day from organogenesis period day 7 to 17. At adolescence, some IF-MF-exposed mice were further divided into exposure, recovery, and sham-exposure groups. The adolescent-exposure groups were exposed again to IF-MF from postnatal day 27 to 48. The expression of mRNA in the hippocampi was examined using a real-time RT-PCR method, and microglia activation was examined by immunohistochemical analysis. The expression levels of NR1 and NR2B as well as transcription factors (CaMKIV, CREB1), inflammatory mediators (COX2, IL-1 β,TNF-α), and the oxidative stress marker heme-oxygenase (HO)-1 were significantly increased in the IF-MF-exposed mice, compared with the control group, in the 7-week-old mice, but not in the 3-week-old mice. Microglia activation was not different between the control and other groups. This study provides the first evidence that early exposure to IF-MF reversibly affects the NMDA receptor, its related signaling pathways, and inflammatory mediators in the hippocampus of young adult mice; these changes are transient and recover after termination of exposure without histopathological changes.
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影响因子：
2.9
作者：
Day, Heidi E. W.;Kryskow, Elisa M.;Nyhuis, Tara J.;Herlihy, Lauren;Campeau, Serge
通讯作者：
Campeau, Serge
影响因子：
3.7
作者：
He YL;Liu DD;Fang YJ;Zhan XQ;Yao JJ;Mei YA
通讯作者：
Mei YA
DOI：
10.1080/10803548.2006.11076678
发表时间：
2006-01-01
影响因子：
2.4
作者：
Decat, Gilbert;Deckx, Leo;Jonlet, Florence
通讯作者：
Jonlet, Florence
影响因子：
1.9
作者：
Miyakoshi, Junji;Horiuchi, Erni;Sakurai, Tomonori
通讯作者：
Sakurai, Tomonori
DOI：
10.1073/pnas.75.12.6314
发表时间：
1978-01-01
影响因子：
11.1
作者：
BAWIN, SM;ADEY, WR;SABBOT, IM
通讯作者：
SABBOT, IM