Conditioned fear inhibits c-fos mRNA expression in the central extended amygdala.
Conditioned fear inhibits c-fos mRNA expression in the central extended amygdala.
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DOI：
10.1016/j.brainres.2008.06.085
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发表时间：
2008-09-10
期刊：
影响因子：
2.9
通讯作者：
Campeau, Serge
中科院分区：
文献类型：
--
作者：
Day, Heidi E. W.;Kryskow, Elisa M.;Nyhuis, Tara J.;Herlihy, Lauren;Campeau, Serge
我们以前已经表明，无条件的应激抑制杏仁核中央核（CEAl/C）和终纹床核（BSTov），形成中央扩展杏仁核的一部分的卵圆分裂的外侧/囊状分裂的神经元。目前的研究调查是否条件性恐惧抑制c-fos mRNA在这些地区的表达。训练雄性大鼠将视觉刺激（光）与足电击相关联或单独暴露于光。训练后，将动物放回装置中，2小时后通过导管远程注射安非他明（2 mg/kg i. p.），以诱导c-fos mRNA并允许测量表达的抑制。然后在30分钟内向大鼠提供15种视觉刺激。正如预期的那样，没有注射安非他明的恐惧条件动物在中央延伸杏仁核中的c-fos mRNA水平极低。相比之下，只接受光照训练（没有恐惧条件反射）并注射安非他明的动物在CEA 1/c和BSTov中具有高水平的c-fos mRNA。与非条件动物相比，经历恐惧调节并在安非他明注射后再次暴露于条件刺激的动物在BSTov和CEAl/c中的c-fos mRNA水平显著降低。这些数据表明，条件性恐惧可以抑制中央延伸杏仁核的神经元。因为这些神经元是GABA能的，并投射到内侧CEA（杏仁输出区），这可能是一种新的机制，从而条件性恐惧增强杏仁输出。
We have shown previously that unconditioned stressors inhibit neurons of the lateral/capsular division of the central nucleus of the amygdala (CEAl/c) and oval division of the bed nucleus of the stria terminalis (BSTov), which form part of the central extended amygdala. The current study investigated whether conditioned fear inhibits c-fos mRNA expression in these regions. Male rats were trained either to associate a visual stimulus (light) with footshock or were exposed to the light alone. After training, animals were replaced in the apparatus, and 2 hours later injected remotely, via a catheter, with amphetamine (2 mg/kg i.p.), to induce c-fos mRNA and allow inhibition of expression to be measured. The rats were then presented with 15 visual stimuli over a 30 minute period. As expected, fear conditioned animals that were not injected with amphetamine, had extremely low levels of c-fos mRNA in the central extended amygdala. In contrast, animals that were trained with the light alone (no fear conditioning) and were injected with amphetamine had high levels of c-fos mRNA in the CEAl/c and BSTov. Animals that underwent fear-conditioning, and were re-exposed to the conditioned stimulus after amphetamine injection had significantly reduced levels of c-fos mRNA in both the BSTov and CEAl/c, compared to the non-conditioned animals. These data suggest that conditioned fear can inhibit neurons of the central extended amygdala. Because these neurons are GABAergic, and project to the medial CEA (an amygdaloid output region), this may be a novel mechanism whereby conditioned fear potentiates amygdaloid output.
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