Kinetics of the Cellular and Transcriptomic Response to Eimeria maxima in Relatively Resistant and Susceptible Chicken Lines.
Kinetics of the Cellular and Transcriptomic Response to Eimeria maxima in Relatively Resistant and Susceptible Chicken Lines.
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DOI：
10.3389/fimmu.2021.653085
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发表时间：
2021
影响因子：
7.3
通讯作者：
Vervelde L
中科院分区：
文献类型：
--
作者：
Bremner A;Kim S;Morris KM;Nolan MJ;Borowska D;Wu Z;Tomley F;Blake DP;Hawken R;Kaiser P;Vervelde L
巨型艾美耳球虫是鸡球虫病的常见原因，这种疾病对家禽生产有巨大的经济影响。对E.最大值和有助于在鸡品种之间和来源于单一鸡品种的同类系之间观察到的不同抗性水平的特定机制。本研究的目的是确定两个白色来航鸡近交系的免疫反应动力学的差异，这两个近交系对E.马克西姆用E.在感染后2、4、6和8天（DPI）进行空肠组织的最大值和转录组分析。RNA-Seq分析揭示了两种细胞系之间感染后细胞因子转录应答的速度和幅度的差异。特别地，与系15 I（在6dpi）相比，系C.B12（在4dpi）中空肠中IFN-γ和IL-10转录物表达更早地增加。C.B12系鸡在感染后4天表现出空肠IFNG和IL 10 mRNA的增加，而在15系鸡中，I转录延迟，但增加的程度更大。RT-qPCR和ELISA证实了转录组学研究的结果。血清IL-10水平与E.与品系C.B12鸡相比，品系15 I中的最大复制。总的来说，这些发现表明IFN-γ和IL-10反应的早期诱导，以及免疫相关基因，包括通过RNA-Seq鉴定的4 dpi时的IL 21，可能是抵抗E.马克西姆
Eimeria maxima is a common cause of coccidiosis in chickens, a disease that has a huge economic impact on poultry production. Knowledge of immunity to E. maxima and the specific mechanisms that contribute to differing levels of resistance observed between chicken breeds and between congenic lines derived from a single breed of chickens is required. This study aimed to define differences in the kinetics of the immune response of two inbred lines of White Leghorn chickens that exhibit differential resistance (line C.B12) or susceptibility (line 15I) to infection by E. maxima. Line C.B12 and 15I chickens were infected with E. maxima and transcriptome analysis of jejunal tissue was performed at 2, 4, 6 and 8 days post-infection (dpi). RNA-Seq analysis revealed differences in the rapidity and magnitude of cytokine transcription responses post-infection between the two lines. In particular, IFN-γ and IL-10 transcript expression increased in the jejunum earlier in line C.B12 (at 4 dpi) compared to line 15I (at 6 dpi). Line C.B12 chickens exhibited increases of IFNG and IL10 mRNA in the jejunum at 4 dpi, whereas in line 15I transcription was delayed but increased to a greater extent. RT-qPCR and ELISAs confirmed the results of the transcriptomic study. Higher serum IL-10 correlated strongly with higher E. maxima replication in line 15I compared to line C.B12 chickens. Overall, the findings suggest early induction of the IFN-γ and IL-10 responses, as well as immune-related genes including IL21 at 4 dpi identified by RNA-Seq, may be key to resistance to E. maxima.
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影响因子：
2.1
作者：
Haritova, A. M.;Stanilova, S. A.
通讯作者：
Stanilova, S. A.
影响因子：
4.4
作者：
Del Cacho E;Gallego M;Lillehoj HS;Quílez J;Lillehoj EP;Ramo A;Sánchez-Acedo C
通讯作者：
Sánchez-Acedo C
影响因子：
6.7
作者：
Blake DP;Billington KJ;Copestake SL;Oakes RD;Quail MA;Wan KL;Shirley MW;Smith AL
通讯作者：
Smith AL
影响因子：
4
作者：
Clark EL;Macdonald SE;Thenmozhi V;Kundu K;Garg R;Kumar S;Ayoade S;Fornace KM;Jatau ID;Moftah A;Nolan MJ;Sudhakar NR;Adebambo AO;Lawal IA;Álvarez Zapata R;Awuni JA;Chapman HD;Karimuribo E;Mugasa CM;Namangala B;Rushton J;Suo X;Thangaraj K;Srinivasa Rao AS;Tewari AK;Banerjee PS;Dhinakar Raj G;Raman M;Tomley FM;Blake DP
通讯作者：
Blake DP
影响因子：
1.8
作者：
Jeurissen, SHM;Janse, EM;Vervelde, L
通讯作者：
Vervelde, L