HEPA filters of portable air cleaners as a tool for the surveillance of SARS-CoV-2.

HEPA filters of portable air cleaners as a tool for the surveillance of SARS-CoV-2.
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DOI:
10.1111/ina.13109
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发表时间:
2022-09
期刊:
影响因子:
5.8
通讯作者:
de la Fuente, Jose
de la Fuente, Jose
中科院分区:
环境科学与生态学2区
文献类型:
--
作者:
Fernandez de Mera, Isabel G.;Granda, Carmen;Villanueva, Florentina;Sanchez-Sanchez, Marta;Moraga-Fernandez, Alberto;Gortazar, Christian;de la Fuente, Jose

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关于室内气溶胶中SARS-CoV-2的鉴定研究已经在医院病房进行,在非医疗环境中也进行了较小程度的研究。在这些研究中,人们已经感染了SARS-CoV-2。然而,在本研究中,我们调查了位于明显健康人群所在地的便携式空气净化器(PAC)中的HEPA过滤器中SARS-CoV-2的存在,以防止可能的爆发。开发并验证了一种检测HEPA过滤器中是否存在SARS-CoV-2 RNA的方法。这项研究在三个室内环境中进行了13周:学校、托儿所和社会健康中心的一个家庭,所有这些都在西班牙的真实的城。在HEPA过滤器和这些室内空间的其他表面上进行了SARS-CoV-2存在的环境监测,以便在无症状人群中进行选择性筛查。目的是在早期阶段限制疾病的爆发。一个HEPA过滤器在社会健康中心检测呈阳性。在对居民和医护人员进行SARS-CoV-2的RT-PCR分析后,一名工作人员检测呈阳性。因此,这项研究提供了HEPA过滤器中捕获含病毒气溶胶的直接证据,以及使用这些PAC进行SARS-CoV-2环境监测的可能性,同时它们可以去除空气中的气溶胶并捕获病毒。
Studies about the identification of SARS‐CoV‐2 in indoor aerosols have been conducted in hospital patient rooms and to a lesser extent in nonhealthcare environments. In these studies, people were already infected with SARS‐CoV‐2. However, in the present study, we investigated the presence of SARS‐CoV‐2 in HEPA filters housed in portable air cleaners (PACs) located in places with apparently healthy people to prevent possible outbreaks. A method for detecting the presence of SARS‐CoV‐2 RNA in HEPA filters was developed and validated. The study was conducted for 13 weeks in three indoor environments: school, nursery, and a household of a social health center, all in Ciudad Real, Spain. The environmental monitoring of the presence of SARS‐CoV‐2 was conducted in HEPA filters and other surfaces of these indoor spaces for a selective screening in asymptomatic population groups. The objective was to limit outbreaks at an early stage. One HEPA filter tested positive in the social health center. After analysis by RT‐PCR of SARS‐CoV‐2 in residents and healthcare workers, one worker tested positive. Therefore, this study provides direct evidence of virus‐containing aerosols trapped in HEPA filters and the possibility of using these PACs for environmental monitoring of SARS‐CoV‐2 while they remove airborne aerosols and trap the virus.
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