Universal quantification of structurally diverse natural products using an evaporative light scattering detector.
Universal quantification of structurally diverse natural products using an evaporative light scattering detector.
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DOI：
10.1021/np300034c
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发表时间：
2012-04-27
影响因子：
5.1
通讯作者：
Bugni TS
中科院分区：
文献类型：
--
作者：
Adnani N;Michel CR;Bugni TS
天然产物的绝对定量缺乏良好的方法，限制了高通量筛选的准确性。目前使用的许多定量方法要么太慢，要么不适合天然产物的结构多样性。低温蒸发光散射检测器（ELSD-LT）的最新发展克服了ELSD的几个历史局限性，包括分析物分解和低灵敏度。首先，ELSD已用于相对定量和检测缺乏UV发色团的化合物。在这项研究中，我们采用ELSD-LT的天然产物的绝对定量。使用加权最小二乘分析构建了一组不同的天然产物和其他化合物的校准曲线。平均校准曲线进行了评估的“通用”定量的天然产物。ELSD-LT硬件和参数的优化提高了灵敏度和通量，并建立了ELSD-LT用于定量大型天然产物文库的实用性。
A lack of good methods for absolute quantification of natural products has limited the accuracy of high-throughput screening. Many currently used methods for quantification are either too slow or not amenable to the structural diversity of natural products. Recent developments in low-temperature evaporative light scattering detectors (ELSD-LT) have overcome several historical limitations of ELSDs, including analyte decomposition and low sensitivity. Primarily, ELSDs have been used for relative quantification and detection of compounds that lack a UV chromophore. In this study, we employ an ELSD-LT for absolute quantification of natural products. Calibration curves were constructed using a weighted least-squares analysis for a diverse set of natural products and other compounds. An average calibration curve was evaluated for the “universal” quantification of natural products. Optimization of ELSD-LT hardware and parameters improved sensitivity and throughput and established the utility of ELSD-LT for quantification of large natural product libraries.
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DOI：
10.1021/ci0200467
发表时间：
2003-01-01
期刊：
JOURNAL OF CHEMICAL INFORMATION AND COMPUTER SCIENCES
影响因子：
--
作者：
Feher, M;Schmidt, JM
通讯作者：
Schmidt, JM
影响因子：
5.1
作者：
Johnson, Tyler A.;Morgan, Micaela V. C.;Crews, Phillip
通讯作者：
Crews, Phillip
影响因子：
5.1
作者：
Robinson, Sarah J.;Hoobler, Eric K.;Riener, Michelle;Loveridge, Steven T.;Tenney, Karen;Valeriote, Frederick A.;Holman, Theodore R.;Crews, Phillip
通讯作者：
Crews, Phillip
影响因子：
13.1
作者：
Kohler, M;Haerdi, W;Veuthey, JL
通讯作者：
Veuthey, JL
影响因子：
3.8
作者：
Kibbey, CE
通讯作者：
Kibbey, CE