Intra-specific diversity of Serratia marcescens in Anopheles mosquito midgut defines Plasmodium transmission capacity.
Intra-specific diversity of Serratia marcescens in Anopheles mosquito midgut defines Plasmodium transmission capacity.
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DOI：
10.1038/srep01641
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发表时间：
2013
影响因子：
4.6
通讯作者：
Kanuka, Hirotaka
中科院分区：
文献类型：
--
作者：
Bando, Hironori;Okado, Kiyoshi;Guelbeogo, Wamdaogo M.;Badolo, Athanase;Aonuma, Hiroka;Nelson, Bryce;Fukumoto, Shinya;Xuan, Xuenan;Sagnon, N'Fale;Kanuka, Hirotaka
疟疾传播的一个关键阶段发生在按蚊中肠，此时疟疾寄生虫疟原虫与血液一起摄入，首先与肠道上皮表面接触。为了了解中肠环境中的反应机制，包括受居民微生物群对疟原虫的影响，我们专注于中肠细菌物种的种内变异，这种变异赋予蚊子传播疟疾的能力多样性。从布基纳法索实验室饲养的蚊子或野生种群中分离的粘质沙雷氏菌在其细胞和结构特征方面表现出很大的表型变异。重要的是，这种变异与其抑制蚊子中肠内疟原虫发育的能力直接相关。此外，粘质沙雷氏菌赋予的这种抗疟原虫功能需要鞭毛生物合成途径的表达增加，该途径由运动性主调节操纵子flhDC调节。这些发现指出了通过对蚊子体内中肠细菌进行遗传操作来控制疟疾的新策略。
A critical stage in malaria transmission occurs in the Anopheles mosquito midgut, when the malaria parasite, Plasmodium, ingested with blood, first makes contact with the gut epithelial surface. To understand the response mechanisms within the midgut environment, including those influenced by resident microbiota against Plasmodium, we focus on a midgut bacteria species' intra-specific variation that confers diversity to the mosquito's competency for malaria transmission. Serratia marcescens isolated from either laboratory-reared mosquitoes or wild populations in Burkina Faso shows great phenotypic variation in its cellular and structural features. Importantly, this variation is directly correlated with its ability to inhibit Plasmodium development within the mosquito midgut. Furthermore, this anti-Plasmodium function conferred by Serratia marcescens requires increased expression of the flagellum biosynthetic pathway that is modulated by the motility master regulatory operon, flhDC. These findings point to new strategies for controlling malaria through genetic manipulation of midgut bacteria within the mosquito.
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影响因子：
32.4
作者：
Frolet, Cecile;Thoma, Martine;Levashina, Elena A.
通讯作者：
Levashina, Elena A.
影响因子：
2.7
作者：
Chavshin, Ali Reza;Oshaghi, Mohammad Ali;Ghoorchian, Sadigheh
通讯作者：
Ghoorchian, Sadigheh
影响因子：
2.1
作者：
Gonzalez-Ceron, L;Santillan, F;Hernandez-Avila, JE
通讯作者：
Hernandez-Avila, JE
影响因子：
2.6
作者：
Grossman, GL;Rafferty, CS;Benedict, MQ
通讯作者：
Benedict, MQ
影响因子：
64.8
作者：
Catteruccia, F;Nolan, T;Crisanti, A
通讯作者：
Crisanti, A