Salmonella enterica serovar Typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by Lactobacillus rhamnosus GG.
Salmonella enterica serovar Typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by Lactobacillus rhamnosus GG.
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DOI：
10.1186/1757-4749-1-14
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发表时间：
2009-07-09
期刊：
影响因子：
4.2
通讯作者：
Bhunia AK
中科院分区：
文献类型：
--
作者：
Burkholder KM;Bhunia AK
生理应激可改变宿主肠上皮对肠道病原体感染的易感性。本研究对鼠伤寒沙门氏菌的细胞毒作用、粘附和侵袭能力进行了研究。鼠伤寒沙门氏菌）对暴露于热应激（41 ° C，1 h）的Caco-2细胞的影响。益生菌已显示在非应激条件下减少病原体与上皮的相互作用，并且在感染期间可能对上皮活力具有显著影响;然而，益生菌在生理应激下对病原体与上皮细胞相互作用的影响尚不清楚。因此，我们研究了鼠李糖乳杆菌GG和加氏乳杆菌对沙门氏菌粘附和沙门氏菌诱导的细胞毒性的Caco-2细胞受到热应激的影响。热应激可增强S.鼠伤寒沙门氏菌（P = 0.0001）和非致病性E. coliK12（P = 0.004）对Caco-2细胞的敏感性增加，并导致细胞单层对S.鼠伤寒粘附（P = 0.001）。热应激对Caco-2细胞释放的炎性细胞因子没有显著影响，尽管暴露于S。鼠伤寒沙门氏菌导致IL-6和IL-8的产生增加超过80%。封锁S。在暴露于沙门氏菌之前，鼠伤寒沙门氏菌与抗ShdA抗体降低了与非应激和热应激Caco-2细胞的粘附（P = 0.01）。Caco-2细胞预暴露于L. rhamnosus GG显著降低热应激时沙门氏菌诱导的细胞毒性（P = 0.001）和沙门氏菌对Caco-2细胞的粘附（P = 0.001），而L.加塞利没有效果。结果表明，热应激增加了肠上皮Caco-2细胞对沙门氏菌粘附的敏感性，并增加了沙门氏菌在感染过程中的细胞毒作用。使用L。作为益生菌的鼠李糖乳杆菌GG可以降低上皮细胞应激期间感染的严重性。热应力增加对S.鼠伤寒定殖和L. rhamnosus GG限制了感染的严重程度仍有待阐明。
Physiological stressors may alter susceptibility of the host intestinal epithelium to infection by enteric pathogens. In the current study, cytotoxic effect, adhesion and invasion of Salmonella enterica serovar Typhimurium (S. Typhimurium) to Caco-2 cells exposed to thermal stress (41°C, 1 h) was investigated. Probiotic bacteria have been shown to reduce interaction of pathogens with the epithelium under non-stress conditions and may have a significant effect on epithelial viability during infection; however, probiotic effect on pathogen interaction with epithelial cells under physiological stress is not known. Therefore, we investigated the influence of Lactobacillus rhamnosus GG and Lactobacillus gasseri on Salmonella adhesion and Salmonella-induced cytotoxicity of Caco-2 cells subjected to thermal stress. Thermal stress increased the cytotoxic effect of both S. Typhimurium (P = 0.0001) and nonpathogenic E. coli K12 (P = 0.004) to Caco-2 cells, and resulted in greater susceptibility of cell monolayers to S. Typhimurium adhesion (P = 0.001). Thermal stress had no significant impact on inflammatory cytokines released by Caco-2 cells, although exposure to S. Typhimurium resulted in greater than 80% increase in production of IL-6 and IL-8. Blocking S. Typhimurium with anti-ShdA antibody prior to exposure of Salmonella decreased adhesion (P = 0.01) to non-stressed and thermal-stressed Caco-2 cells. Pre-exposure of Caco-2 cells to L. rhamnosus GG significantly reduced Salmonella-induced cytotoxicity (P = 0.001) and Salmonella adhesion (P = 0.001) to Caco-2 cells during thermal stress, while L. gasseri had no effect. Results suggest that thermal stress increases susceptibility of intestinal epithelial Caco-2 cells to Salmonella adhesion, and increases the cytotoxic effect of Salmonella during infection. Use of L. rhamnosus GG as a probiotic may reduce the severity of infection during epithelial cell stress. Mechanisms by which thermal stress increases susceptibility to S. Typhimurium colonization and by which L. rhamnosus GG limits the severity of infection remain to be elucidated.
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影响因子：
15.9
作者：
Gewirtz, AT;Rao, AS;Neish, AS
通讯作者：
Neish, AS
影响因子：
4.4
作者：
Burkholder, K. M.;Thompson, K. L.;Patterson, J. A.
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影响因子：
2.6
作者：
Fajdiga, S.;Koninkx, J. F. J. G.;Marinsek-Logar, R.
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Marinsek-Logar, R.
影响因子：
4.4
作者：
Hinton, A;Buhr, RJ;Ingram, KD
通讯作者：
Ingram, KD
影响因子：
6.4
作者：
Gupta, A;Fontana, J;Angulo, FJ
通讯作者：
Angulo, FJ