Myb10-D confers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat.
Myb10-D confers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat.
复制标题
Myb10-D 通过调节小麦 ABA 生物合成途径中的 NCED 赋予 PHS-3D 对收获前发芽的抗性
DOI：
10.1111/nph.17312
复制
发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Lang J;Fu Y;Zhou Y;Cheng M;Deng M;Li M;Zhu T;Yang J;Guo X;Gui L;Li L;Chen Z;Yi Y;Zhang L;Hao M;Huang L;Tan C;Chen G;Jiang Q;Qi P;Pu Z;Ma J;Liu Z;Liu Y;Luo MC;Wei Y;Zheng Y;Wu Y;Liu D;Wang J
收获前发芽（PHS），即收获前谷物的发芽，是导致小麦产量和品质损失的严重问题。在这里，我们将合成六倍体小麦的PHS抗性基因PHS-3D定位到2.4 Mb的存在-不存在变异（PAV）区域，并通过综合组学和功能分析发现其抗性效应归因于多效性Myb 10-D。在全球262个小麦株系和16个节节麦中，在PAV区域检测到3种单倍型，含Myb 10 ‐D的小麦株系的发芽率比其他株系低约40%。转录组和代谢组分析表明，Myb 10 ‐D影响类黄酮和脱落酸（阿坝）生物合成途径中基因的转录，导致转基因小麦品系中类黄酮和阿坝的增加。Myb 10-D通过与次生壁MYB-反应元件（SMRE）结合激活9-顺式-环氧类胡萝卜素双加氧酶（NCED），促进小麦种子发育早期阿坝合成。我们揭示了Myb 10 ‐D的新发现的功能通过增强阿坝生物合成来延迟小麦的发芽，从而赋予PHS抗性。携带与粒色和PHS相关的Myb 10 ‐D的PAV将有助于理解和选择白色粒抗PHS小麦品种。
Pre‐harvest sprouting (PHS), the germination of grain before harvest, is a serious problem resulting in wheat yield and quality losses. Here, we mapped the PHS resistance gene PHS‐3D from synthetic hexaploid wheat to a 2.4 Mb presence–absence variation (PAV) region and found that its resistance effect was attributed to the pleiotropic Myb10‐D by integrated omics and functional analyses. Three haplotypes were detected in this PAV region among 262 worldwide wheat lines and 16 Aegilops tauschii, and the germination percentages of wheat lines containing Myb10‐D was approximately 40% lower than that of the other lines. Transcriptome and metabolome profiling indicated that Myb10‐D affected the transcription of genes in both the flavonoid and abscisic acid (ABA) biosynthesis pathways, which resulted in increases in flavonoids and ABA in transgenic wheat lines. Myb10‐D activates 9‐cis‐epoxycarotenoid dioxygenase (NCED) by biding the secondary wall MYB‐responsive element (SMRE) to promote ABA biosynthesis in early wheat seed development stages. We revealed that the newly discovered function of Myb10‐D confers PHS resistance by enhancing ABA biosynthesis to delay germination in wheat. The PAV harboring Myb10‐D associated with grain color and PHS will be useful for understanding and selecting white grained PHS resistant wheat cultivars.
登录
查看更多内容
影响因子：
3.3
作者：
Imtiaz, Muhammad;Ogbonnaya, Francis C.;van Ginkel, Maarten
通讯作者：
van Ginkel, Maarten
影响因子：
3.1
作者：
MCFADDEN, ES;SEARS, ER
通讯作者：
SEARS, ER
影响因子：
--
作者：
GORDON, IL
通讯作者：
GORDON, IL
影响因子：
2
作者：
Liu, DC;Lan, XJ;Yen, C
通讯作者：
Yen, C
影响因子：
1.2
作者：
DEPAUW, RM;MCCAIG, TN
通讯作者：
MCCAIG, TN