The steroid hormone ecdysone functions with intrinsic chromatin remodeling factors to control female germline stem cells in Drosophila.
The steroid hormone ecdysone functions with intrinsic chromatin remodeling factors to control female germline stem cells in Drosophila.
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DOI：
10.1016/j.stem.2010.10.001
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发表时间：
2010-11-05
期刊：
影响因子：
23.9
通讯作者：
Drummond-Barbosa, Daniela
中科院分区：
文献类型：
--
作者：
Ables, Elizabeth T.;Drummond-Barbosa, Daniela
类固醇激素是已知的多种正常和癌组织的系统调节剂;然而，它们是否或如何影响成体干细胞的命运和功能尚不清楚。在果蝇卵巢中，胰岛素信号调节生殖系干细胞（GSC）的增殖和自我更新，然而，尽管有证据表明，其他系统性因素控制GSC的活动，这些仍然在很大程度上是未知的。在这里，我们报告说，蜕皮激素，结构上与哺乳动物性类固醇激素，直接调节成人GSC增殖和自我更新独立的胰岛素信号。蜕皮激素通过与染色质重塑因子ISWI和Nurf 301的功能性相互作用来控制GSC，ISWI是GSC命运和活性所需的内在表观遗传因子，Nurf 301是含ISWI的GNF染色质重塑复合物的最大亚基。我们的研究结果支持系统性类固醇激素和内在染色质重塑机制之间的联系，作为促进成体干细胞自我更新所需的广泛转录程序的潜在机制。
Steroid hormones are known systemic regulators of multiple normal and cancerous tissues; however, whether or how they impact the fate and function of adult stem cells is unclear. In the Drosophila ovary, insulin signals modulate the proliferation and self-renewal of germline stem cells (GSCs), yet despite evidence that additional systemic factors control GSC activity, these have remained largely unknown. Here, we report that ecdysone, a steroid hormone structurally related to mammalian sex steroids, directly regulates adult GSC proliferation and self-renewal independently of insulin signaling. Ecdysone controls GSCs through a functional interaction with the chromatin remodeling factors ISWI, an intrinsic epigenetic factor required for GSC fate and activity, and Nurf301, the largest subunit of the ISWI-containing NURF chromatin remodeling complex. Our findings support a link between systemic steroid hormones and the intrinsic chromatin remodeling machinery as a potential mechanism to promote broad transcriptional programs required for adult stem cell self-renewal.
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DOI：
10.1073/pnas.0809144106
发表时间：
2009-01-27
影响因子：
11.1
作者：
Hsu, Hwei-Jan;Drummond-Barbosa, Daniela
通讯作者：
Drummond-Barbosa, Daniela
影响因子：
16
作者：
Deuring, R;Fanti, L;Tamkun, JW
通讯作者：
Tamkun, JW
影响因子：
64.8
作者：
Asselin-Labat, Marie-Liesse;Vaillant, Francois;Visvader, Jane E.
通讯作者：
Visvader, Jane E.
DOI：
10.1073/pnas.061437798
发表时间：
2001-03-27
影响因子：
11.1
作者：
Ghbeish, N;Tsai, CC;McKeown, M
通讯作者：
McKeown, M
影响因子：
10.5
作者：
Badenhorst, P;Xiao, H;Wu, C
通讯作者：
Wu, C