DELAY OF GERMINATION 1, the Master Regulator of Seed Dormancy, Integrates the Regulatory Network of Phytohormones at the Transcriptional Level to Control Seed Dormancy.
DELAY OF GERMINATION 1, the Master Regulator of Seed Dormancy, Integrates the Regulatory Network of Phytohormones at the Transcriptional Level to Control Seed Dormancy.
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DOI：
10.3390/cimb44120423
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发表时间：
2022-12-08
影响因子：
3.1
通讯作者：
Xiang, Yong
中科院分区：
文献类型：
--
作者：
Li, Qiujia;Chen, Xi;Zhang, Shengnan;Shan, Siyao;Xiang, Yong
种子休眠是决定种子萌发时间的一个重要的适应性性状，受植物激素和遗传因素的内源控制。发芽延迟1（DOG 1）是休眠的重要遗传调节因子，显著影响许多阿坝和GA代谢基因的表达。然而，DOG 1是否能够影响其他植物激素相关基因的表达仍然是未知的。在这里，我们通过使用全转录组测序（RNA-seq）全面调查了所有有充分记录的可能在dog 1 -2干燥或吸胀种子中受到影响的与种子相关的基因。我们发现DOG 1可以系统地控制植物激素相关基因的表达。在dog 1 -2背景下，脱落酸（阿坝）和吲哚乙酸（IAA）的内源信号强度明显降低，而赤霉素（GA）、油菜素内酯（BR）和细胞分裂素（CK）的内源信号强度显著增加，这可能与dog 1 -2的休眠缺陷表型有关。总的来说，我们的数据突出了DOG 1在平衡植物激素相关基因表达方面的作用，并提供了鼓舞人心的证据，即DOG 1可能整合植物激素网络来控制种子休眠。
Seed dormancy, an important adaptive trait that governs germination timing, is endogenously controlled by phytohormones and genetic factors. DELAY OF GERMINATION 1 (DOG1) is the vital genetic regulator of dormancy, significantly affecting the expression of numerous ABA and GA metabolic genes. However, whether DOG1 could influence the expression of other phytohormone-related genes is still unknown. Here, we comprehensively investigated all well-documented hormone-related genes which might be affected in dog1–2 dry or imbibed seeds by using whole-transcriptome sequencing (RNA-seq). We found that DOG1 could systematically control the expression of phytohormone-related genes. An evident decrease was observed in the endogenous signal intensity of abscisic acid (ABA) and indole-3-acetic acid (IAA), while a dramatic increase appeared in that of gibberellins (GA), brassinosteroids (BR), and cytokinin (CK) in the dog1–2 background, which may contribute considerably to its dormancy-deficient phenotype. Collectively, our data highlight the role of DOG1 in balancing the expression of phytohormone-related genes and provide inspirational evidence that DOG1 may integrate the phytohormones network to control seed dormancy.
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影响因子：
27.5
作者：
Berriri S;Gangappa SN;Kumar SV
通讯作者：
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影响因子：
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作者：
Dekkers, Bas J. W.;He, Hanzi;Bentsink, Leonie
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影响因子：
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作者：
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DOI：
10.1073/pnas.1608827113
发表时间：
2016-11-29
影响因子：
11.1
作者：
Fedak, Halina;Palusinska, Malgorzata;Swiezewski, Szymon
通讯作者：
Swiezewski, Szymon
DOI：
10.1073/pnas.1600558113
发表时间：
2016-04-12
影响因子：
11.1
作者：
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通讯作者：
Bradford, Kent J.