Practical uses for ecdysteroids in mammals including humans: an update.
Practical uses for ecdysteroids in mammals including humans: an update.
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DOI：
10.1093/jis/3.1.7
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Dinan L
中科院分区：
文献类型：
--
作者：
Lafont R;Dinan L
蜕皮甾类被广泛用作基于昆虫蜕皮甾类受体的基因开关系统的诱导剂，并且感兴趣的基因被置于蜕皮甾类反应元件的控制下。我们在这里回顾这些系统，目前主要用于体外培养细胞，以分析广泛的基因的作用，但预计将代表未来的基因治疗策略的基础。这些事态发展提出了几个问题，将详细讨论这些问题。首先，蜕皮类固醇在哺乳动物（包括人类）中的代谢命运知之甚少，并且蜕皮类固醇的快速分解可能阻碍其作为体内诱导剂的使用。事实上，第二组问题的出现要早得多，早在60年代初，伯德特就对蜕皮类固醇对哺乳动物的药理作用进行了开创性的“异嗜性”研究。这些和随后的研究显示了广泛的影响，其中大多数对生物体有益（例如，低血糖、低胆固醇血症、合成代谢）。这些影响进行了审查和批判性分析，并提出了一些假设来解释所涉及的假定机制。所有这些药理学作用已经导致开发了广泛的含蜕皮类固醇的制剂，其主要用于人（或马或狗）的合成代谢和/或“适应性”特性。以同样的方式，越来越多的专利已经被保藏，涉及蜕皮甾体在许多医学或化妆品领域中的各种有益效果，这使得蜕皮甾体对于几种实际用途来说是非常有吸引力的候选物。人们可能会问，是否所有这些药理作用是兼容的蜕皮类固醇诱导基因开关的基因治疗的发展，以及蜕皮类固醇是否应归类为兴奋剂物质。20-羟基蜕皮激素2-脱氧-20-羟基蜕皮激素2-脱氧蜕皮激素双酰肼家蚕EcR Choristoneura fumiferana EcR Choristoneura fumiferana USP中国仓鼠卵巢巨细胞病毒DNA结合域果蝇EcR蜕皮激素蜕皮激素受体蜕皮激素反应元件有效半时间雌激素反应元件糖皮质激素受体糖皮质激素反应元件人胚肾烟芽夜蛾EcR配体结合域鼠甾酮A蛋白激酶A Polypodine B ponasterone A过氧化物酶体增殖物激活受体视黄酸受体维甲酸X受体甲状腺受体超气门维生素D受体血管内皮生长因子
Ecdysteroids are widely used as inducers for gene-switch systems based on insect ecdysteroid receptors and genes of interest placed under the control of ecdysteroid-response elements. We review here these systems, which are currently mainly used in vitro with cultured cells in order to analyse the role of a wide array of genes, but which are expected to represent the basis for future gene therapy strategies. Such developments raise several questions, which are addressed in detail. First, the metabolic fate of ecdysteroids in mammals, including humans, is only poorly known, and the rapid catabolism of ecdysteroids may impede their use as in vivo inducers. A second set of questions arose in fact much earlier with the pioneering “heterophylic” studies of Burdette in the early sixties on the pharmacological effects of ecdysteroids on mammals. These and subsequent studies showed a wide range of effects, most of them being beneficial for the organism (e.g. hypoglycaemic, hypocholesterolaemic, anabolic). These effects are reviewed and critically analysed, and some hypotheses are proposed to explain the putative mechanisms involved. All of these pharmacological effects have led to the development of a wide array of ecdysteroid-containing preparations, which are primarily used for their anabolic and/or “adaptogenic” properties on humans (or horses or dogs). In the same way, increasing numbers of patents have been deposited concerning various beneficial effects of ecdysteroids in many medical or cosmetic domains, which make ecdysteroids very attractive candidates for several practical uses. It may be questioned whether all these pharmacological actions are compatible with the development of ecdysteroid-inducible gene switches for gene therapy, and also if ecdysteroids should be classified among doping substances. 20-hydroxyecdysone 2-deoxy-20-hydroxyecdysone 2-deoxyecdysone bisacylhydrazine Bombyx mori EcR Choristoneura fumiferana EcR Choristoneura fumiferana USP Chinese hamster ovary cytomegalovirus DNA-binding domain Drosophila melanogaster EcR ecdysone ecdysteroid receptor ecdysteroid response element effective half-time oestrogen response element glucocorticoid receptor glucocorticoid response element human embryonic kidney Heliothis virescens EcR ligand binding domain muristerone A protein kinase A polypodine B ponasterone A peroxisome proliferator-activated receptor retinoic acid receptor retinoid X receptor thyroid receptor ultraspiracle vitamin D receptor vascular endothelial growth factor
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影响因子：
6.1
作者：
Bohl, D;Heard, JM
通讯作者：
Heard, JM
影响因子：
4.8
作者：
Albanese, C;Reutens, AT;Pestell, RG
通讯作者：
Pestell, RG
DOI：
10.1016/0742-8413(84)90068-9
发表时间：
1984-01-01
影响因子：
3.9
作者：
CATALAN, RE;ARAGONES, MD;MARTINEZ, AM
通讯作者：
MARTINEZ, AM
影响因子：
0.8
作者：
BIDMON, HJ;SLITER, TJ
通讯作者：
SLITER, TJ
DOI：
10.1016/0006-291x(79)90940-9
发表时间：
1979-01-01
影响因子：
3.1
作者：
CATALAN, RE;ARAGONES, MD;MARTINEZ, AM
通讯作者：
MARTINEZ, AM