A Microtus fortis protein, serum albumin, is a novel inhibitor of Schistosoma japonicum schistosomula.
A Microtus fortis protein, serum albumin, is a novel inhibitor of Schistosoma japonicum schistosomula.
复制标题
东方田鼠蛋白、血清白蛋白是日本血吸虫的新型抑制剂
DOI：
10.1590/0074-0276130659
复制
发表时间：
2013-11
影响因子：
2.8
通讯作者：
Hu WX
中科院分区：
文献类型：
--
作者：
Li R;Wu GJ;Xiong DH;Gong Q;Yu RJ;Hu WX
血吸虫病是一种地方性寄生虫病，吡喹酮是目前用于控制该疾病的唯一药物。实验和流行病学证据强烈表明，东方田鼠（Mf）是日本血吸虫的天然抗性脊椎动物宿主。在本研究中，我们发现Mf血清白蛋白（Mf-albumin）和pcDNA3.1-Mf-albumin条件培养基在96 h内分别导致血吸虫死亡率46.2%和38.7%，显着高于阴性对照（p < 0.05）。我们还发现，与对照动物相比，注射 Mf -白蛋白的小鼠的蠕虫负担减少了 43.5%，每克肝卵减少了 48.1%（p < 0.05）。为了表征清除所涉及的机制，将血吸虫与异硫氰酸荧光素标记的Mf-白蛋白一起孵育，孵育48小时后在血吸虫的肠腔中发现荧光富集效应。接下来，收集来自血吸虫的消化道排泄物并评价Mf-白蛋白对消化道排泄物的敏感性。结果表明，血吸虫消化道排泄物呈难消化性Mf-白蛋白。血吸虫死亡的部分原因可能是由于血吸虫阶段发育过程中消化道排泄物对Mf-白蛋白的消化不足。因此，这些数据表明Mf-白蛋白作为血吸虫病的主要选择力之一的潜力。
Schistosomiasis is an endemic parasite disease and praziquantel is the only drug currently in use to control this disease. Experimental and epidemiological evidence strongly suggests that Microtus fortis ( Mf ) is a naturally resistant vertebrate host of Schistosoma japonicum . In the present study, we found that Mf serum albumin ( Mf -albumin) and the conditioned medium of pcDNA3.1- Mf -albumin caused 46.2% and 38.7% schistosomula death rates in 96 h, respectively, which were significantly higher than that of the negative control (p < 0.05). We also found that mice injected with Mf -albumin had a 43.5% reduction in worm burden and a 48.1% reduction in liver eggs per gram (p < 0.05) in comparison to the control animals. To characterise the mechanisms involved in clearance, schistosomula were incubated with fluorescein isothiocyanate-labelled Mf -albumin and fluorescent enrichment effects were found in the gut lumen of schistosomula after 48 h of incubation. Next, digestive tract excretions from schistosomula were collected and the sensitivity of Mf -albumin to digestive tract excretions was evaluated. The results indicated that schistosomula digestive tract excretions showed indigestibility of Mf -albumin. The death of schistosomula could be partially attributed to the lack of digestion of Mf -albumin by digestive tract excretions during the development of the schistosomula stage. Therefore, these data indicate the potential of Mf -albumin as one of the major selective forces for schistosomiasis.
登录
查看更多内容
影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
--
作者：
Nagel L;Robb T;Forbes MR
通讯作者：
Forbes MR
影响因子：
2.1
作者：
SALAFSKY, B;FUSCO, AC;ELLENBERGER, B
通讯作者：
ELLENBERGER, B
影响因子：
2.9
作者：
Brummer, E;Drasin, T;Stevens, DA
通讯作者：
Stevens, DA
影响因子：
2
作者：
Gold, D;Flescher, E
通讯作者：
Flescher, E