Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis.
Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis.
复制标题
DOI：
10.1016/j.vetmic.2018.02.019
复制
发表时间：
2018-04
影响因子：
3.3
通讯作者：
BRaDP1T consortium
中科院分区：
文献类型：
--
作者：
Bossé JT;Li Y;Sárközi R;Fodor L;Lacouture S;Gottschalk M;Casas Amoribieta M;Angen Ø;Nedbalcova K;Holden MTG;Maskell DJ;Tucker AW;Wren BW;Rycroft AN;Langford PR;BRaDP1T consortium
胸膜肺炎放线杆菌两个新血清型的鉴定。用同源抗血清进行特异性反应的血清学确认。血清变型17和18的荚膜基因座的表征。开发用于分子诊断的PCR。本研究的目的是调查分离的胸膜肺炎放线杆菌先前指定的血清学上的非分型（NT）或作为“K2：07”，不产生血清特异性扩增子的PCR检测。我们使用全基因组测序来鉴定六个先前指定的生物型1 NT和两个生物型1 'K2：O 7'分离株的荚膜（CPS）位点。来自丹麦的胸膜肺炎，以及最近来自加拿大的生物变型2 NT分离株。所有NT分离株具有相同的六基因I型CPS基因座，与血清型2、3、6、7、8、9、11和13共享共同的cpsABC基因。两个'K2：O 7'分离株含有独特的三基因II型CPS基因座，具有与血清型1、4、12、14和15相似的cpsA基因。先前的NT分离株与血清型3、6、8和15共享在erpA和rpsU之间发现的相同O-抗原基因。而“K2：O 7”分离株与血清型7具有相同的O抗原基因，这可能导致其先前的错误鉴定。所有NT和“K2：O 7”分离株仅具有产生ApxII所需的基因（apxIICA结构基因和apxIBD输出基因）。与16种已知血清型相比，针对具有这些新CPS位点的代表性分离株产生的兔多克隆抗血清表现出不同的反应性。血清学和基因组结果表明，分离株构成新的血清型17（以前的NT）和18（以前的“K2：O 7”）。为扩增17型和18型特异性序列而设计的分子诊断引物将有助于对这两种新的A型进行流行病学追踪。胸膜肺炎
Identification of two new serovars of Actinobacillus pleuropneumoniae. Serological confirmation of specific reactivity with homologous antisera. Characterization of the capsule loci of serovars 17 and 18. Development of PCRs for molecular diagnostics. The aim of this study was to investigate isolates of Actinobacillus pleuropneumoniae previously designated serologically either as non-typable (NT) or as ‘K2:07’, which did not produce serovar-specific amplicons in PCR assays. We used whole genome sequencing to identify the capsule (CPS) loci of six previously designated biovar 1 NT and two biovar 1 ‘K2:O7’ isolates of A. pleuropneumoniae from Denmark, as well as a recent biovar 2 NT isolate from Canada. All of the NT isolates have the same six-gene type I CPS locus, sharing common cpsABC genes with serovars 2, 3, 6, 7, 8, 9, 11 and 13. The two ‘K2:O7’ isolates contain a unique three-gene type II CPS locus, having a cpsA gene similar to that of serovars 1, 4, 12, 14 and 15. The previously NT isolates share the same O-antigen genes, found between erpA and rpsU, as serovars 3, 6, 8, and 15. Whereas the ‘K2:O7’ isolates, have the same O-antigen genes as serovar 7, which likely contributed to their previous mis-identification. All of the NT and ‘K2:O7’ isolates have only the genes required for production of ApxII (apxIICA structural genes, and apxIBD export genes). Rabbit polyclonal antisera raised against representative isolates with these new CPS loci demonstrated distinct reactivity compared to the 16 known serovars. The serological and genomic results indicate that the isolates constitute new serovars 17 (previously NT) and 18 (previously ‘K2:O7’). Primers designed for amplification of specific serovar 17 and 18 sequences for molecular diagnostics will facilitate epidemiological tracking of these two new serovars of A. pleuropneumoniae.
登录
查看更多内容
影响因子：
9.4
作者：
Bossé JT;Li Y;Sárközi R;Gottschalk M;Angen Ø;Nedbalcova K;Rycroft AN;Fodor L;Langford PR
通讯作者：
Langford PR
影响因子：
3.3
作者：
FREY, J;BECK, M;NICOLET, J
通讯作者：
NICOLET, J
DOI：
10.1099/00207713-33-3-510
发表时间：
1983-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
POHL, S;BERTSCHINGER, HU;MANNHEIM, W
通讯作者：
MANNHEIM, W
影响因子：
2.4
作者：
Turni, C.;Singh, R.;Blackall, P. J.
通讯作者：
Blackall, P. J.
影响因子：
0.9
作者：
Sarkoezi, Rita;Makrai, Laszlo;Fodor, Laszlo
通讯作者：
Fodor, Laszlo