In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami.

In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami.
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使用延时原子力显微镜对功能化 DNA 折纸上的单分子结合反应进行原位监测。

DOI:
10.1039/c1nr10181a
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发表时间:
2011-06
期刊:
影响因子:
6.7
通讯作者:
Li, Bin*
Li, Bin*
中科院分区:
材料科学2区
文献类型:
--
作者:
Wu, Na;Zhou, Xingfei;Czajkowsky, Daniel M.;Ye, Ming;Zeng, Dongdong;Fu, Yanming;Fan, Chunhai;Hu, Jun;Li, Bin*

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通过结合延时原子力显微镜和DNA折纸原位记录单个生物分子结合事件。与特异性生物素化DNA折纸结合的单个链霉亲和素分子简单地计数为时间的函数,以获得结合率的直接测量。
Individual biomolecular binding events were recorded in situ by combining time-lapse atomic force microscopy and DNA origami. Single streptavidin molecules bound to specifically biotinyated DNA origami were simply counted as a function of time to obtain a direct measure of the binding rate.
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