Multilayer DNA origami packed on a square lattice.
Multilayer DNA origami packed on a square lattice.
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DOI：
10.1021/ja906381y
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发表时间：
2009-11-04
影响因子：
15
通讯作者：
Yan, Hao
中科院分区：
文献类型：
--
作者：
Ke, Yonggang;Douglas, Shawn M.;Liu, Minghui;Sharma, Jaswinder;Cheng, Anchi;Leung, Albert;Liu, Yan;Shih, William M.;Yan, Hao
使用DNA作为结构构建块的分子自组装已被证明是构建日益复杂的纳米级物体和阵列的有效途径。使用引人注目的“支架DNA折纸”策略，Rothemund证明了来自病毒基因组（M13）的长单链DNA可以使用数百个短合成DNA分子作为钉合链折叠成各种定制的二维（2D）形状。最近，我们推广了一种策略来构建定制形状的三维（3D）物体，这些物体形成为限制在蜂窝晶格上的螺旋褶皱层，具有精确控制的尺寸范围从10到100 nm。在这里，我们描述了一种更紧凑的3D折纸设计，在正方形晶格上堆积螺旋层，尽管DNA螺旋密度增加，但可以在一步退火过程中成功折叠成设计尺寸的结构。正方形晶格为设计矩形结构提供了更自然的框架，为更密集的结构提供了选择，并且能够创建比蜂窝晶格更平坦的表面。因此，使设计和构建定制的三维形状从螺旋包装在一个正方形晶格提供了一个一般的基础进步，增加DNA纳米技术的多功能性和范围。
Molecular self-assembly using DNA as a structural building block has proven to be an efficient route to the construction of nanoscale objects and arrays of increasing complexity. Using the remarkable “scaffolded DNA origami” strategy, Rothemund demonstrated that a long single-stranded DNA from a viral genome (M13) can be folded into a variety of custom two-dimensional (2D) shapes using hundreds of short synthetic DNA molecules as staple strands. More recently, we generalized a strategy to build custom-shaped, three-dimensional (3D) objects formed as pleated layers of helices constrained to a honeycomb lattice, with precisely controlled dimensions ranging from 10 to 100 nm. Here we describe a more compact design for 3D origami, with layers of helices packed on a square lattice, that can be folded successfully into structures of designed dimensions in a one-step annealing process, despite the increased density of DNA helices. A square lattice provides a more natural framework for designing rectangular structures, the option for a more densely packed architecture, and the ability to create surfaces that are more flat than is possible with the honeycomb lattice. Thus enabling the design and construction of custom 3D shapes from helices packed on a square lattice provides a general foundational advance for increasing the versatility and scope of DNA nanotechnology.
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影响因子：
14.9
作者：
Douglas SM;Marblestone AH;Teerapittayanon S;Vazquez A;Church GM;Shih WM
通讯作者：
Shih WM
影响因子：
10.8
作者：
Ke, Yonggang;Sharma, Jaswinder;Yan, Hao
通讯作者：
Yan, Hao
影响因子：
56.9
作者：
Lee, KB;Park, SJ;Mrksich, M
通讯作者：
Mrksich, M
影响因子：
64.8
作者：
Douglas, Shawn M.;Dietz, Hendrik;Liedl, Tim;Hoegberg, Bjoern;Graf, Franziska;Shih, William M.
通讯作者：
Shih, William M.
DOI：
10.1126/science.1165831
发表时间：
2009-01-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Sharma J;Chhabra R;Cheng A;Brownell J;Liu Y;Yan H
通讯作者：
Yan H