Genetic Loci Conferring Reducing Sugar Accumulation and Conversion of Cold-Stored Potato Tubers Revealed by QTL Analysis in a Diploid Population.
Genetic Loci Conferring Reducing Sugar Accumulation and Conversion of Cold-Stored Potato Tubers Revealed by QTL Analysis in a Diploid Population.
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通过 QTL 分析揭示二倍体群体中降低冷藏马铃薯块茎糖积累和转化的遗传位点
DOI：
10.3389/fpls.2018.00315
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发表时间：
2018
影响因子：
5.6
通讯作者：
Xie C
中科院分区：
文献类型：
--
作者：
Xiao G;Huang W;Cao H;Tu W;Wang H;Zheng X;Liu J;Song B;Xie C
马铃薯块茎低温贮藏过程中还原糖积累引起的冷致甜化是影响薯类产品品质的重要因素。通过提高储藏温度进行修复(REC)是降低RS的常用措施。然而，CIS和REC都是基因依赖的，其遗传基础仍然不确定。本研究以具有广泛遗传背景的二倍体马铃薯群体为材料，分别在其敏感亲本的第5、6、7染色体和抗性亲本的第5、11染色体上定位了4个可重复的CIS QTL和2个REC QTL，表明这两个性状可能在遗传上是独立的。定位在QTL CIS_E_07-1上的淀粉合成基因AGPS2和与QTL REC_B_05-1共定位的淀粉水解基因GWD也支持这一假说。已确定的QTL的累积效应被证明对CIS和REC有很大且稳定的贡献，并在由一系列品种和育种系组成的自然群体中得到证实。本研究首次确定了不同条件下马铃薯CIS和REC的可重复QTL，并首次阐明了其累积遗传效应，为块茎品质改良提供了理论依据和实用手段。
Cold-induced sweetening (CIS) caused by reducing sugar (RS) accumulation during storage in low temperature in potato tubers is a critical factor influencing the quality of fried potato products. The reconditioning (REC) by arising storage temperature is a common measure to lower down RS. However, both CIS and REC are genotype-dependent and the genetic basis remains uncertain. In the present study, with a diploid potato population with broad genetic background, four reproducible QTL of CIS and two of REC were resolved on chromosomes 5, 6, and 7 of the CIS-sensitive parent and chromosomes 5 and 11 of the CIS-resistant parent, respectively, implying that these two traits may be genetically independent. This hypothesis was also supported by the colocalization of two functional genes, a starch synthesis gene AGPS2 mapped in QTL CIS_E_07-1 and a starch hydrolysis gene GWD colocated with QTL REC_B_05-1. The cumulative effects of identified QTL were proved to contribute largely and stably to CIS and REC and confirmed with a natural population composed of a range of cultivars and breeding lines. The present research identified reproducible QTL for CIS and REC of potato in diverse conditions and elucidated for the first time their cumulative genetic effects, which provides theoretical bases and applicable means for tuber quality improvement.
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影响因子：
3.1
作者：
Liu, Xun;Zhang, Chi;Li, Xiu-Qing
通讯作者：
Li, Xiu-Qing
冷藏马铃薯块茎中转化酶和转化酶抑制剂的相互作用蛋白表明转化酶翻译后调节背后存在蛋白质复合物
影响因子：
6.5
作者：
Lin, Yuan;Liu, Jun;Xie, Conghua
通讯作者：
Xie, Conghua
影响因子：
5.4
作者：
Li, Li;Paulo, Maria-Joao;Strahwald, Josef;Luebeck, Jens;Hofferbert, Hans-Reinhard;Tacke, Eckhart;Junghans, Holger;Wunder, Joerg;Draffehn, Astrid;van Eeuwijk, Fred;Gebhardt, Christiane
通讯作者：
Gebhardt, Christiane
DOI：
10.1073/pnas.0406674102
发表时间：
2005-02-01
影响因子：
11.1
作者：
Mikkelsen, R;Mutenda, KE;Blennow, A
通讯作者：
Blennow, A
影响因子：
5.4
作者：
Feingold, S;Lloyd, J;Lorenzen, J
通讯作者：
Lorenzen, J