Population genetics of Plasmodium falciparum and Plasmodium vivax and asymptomatic malaria in Temotu Province, Solomon Islands.
Population genetics of Plasmodium falciparum and Plasmodium vivax and asymptomatic malaria in Temotu Province, Solomon Islands.
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DOI：
10.1186/1475-2875-12-429
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发表时间：
2013-11-22
期刊：
影响因子：
3
通讯作者：
Cheng Q
中科院分区：
文献类型：
--
作者：
Gray KA;Dowd S;Bain L;Bobogare A;Wini L;Shanks GD;Cheng Q
所罗门群岛的特莫图省正在朝着消灭疟疾的方向前进。2008年进行的一项基线调查显示，该省大多数疟原虫感染是低寄生虫密度和无症状感染。为了更好地了解疟疾传播的机制，对该省恶性疟原虫和间日疟原虫种群的遗传多样性和相互关系进行了研究。用8个恶性疟原虫和7个间日疟原虫微卫星标记成功地对2008年基线调查中收集的45个恶性疟原虫和67个间日疟原虫样本进行了基因分型。对恶性疟原虫和间日疟原虫种群的遗传多样性、亲缘关系和分布进行了分析。恶性疟原虫种群多样性较低，共鉴定出19种单倍型，且具有紧密相关的聚类群，表明其具有克隆性扩张。有趣的是，显性单倍型与发热和高寄生虫密度显著相关。相比之下，间日疟原虫种群高度多样化，发现了58种关系不密切的单倍型。全省不同岛屿间的寄生虫种群表现出较低的遗传分化。恶性疟原虫种群的低多样性和克隆性可能是其对疾病产生临床免疫的部分原因。然而，一种新的恶性疟原虫株的输入可能是导致疾病的主要原因。间日疟原虫种群的高度多样性和株间较低的亲缘关系提示，临床对间日疟原虫的免疫可能通过不同的机制维持。从遗传多样性、种群结构和菌株分布来看，2008年我国恶性疟原虫传播率较低，但间日疟原虫传播率仍较高。这些数据将有助于评估干预措施导致的疟疾传播变化。
Temotu Province, Solomon Islands is progressing toward malaria elimination. A baseline survey conducted in 2008 showed that most Plasmodium infections in the province were of low parasite density and asymptomatic infections. To better understand mechanisms underlying these malaria transmission characteristics genetic diversity and relationships among Plasmodium falciparum and Plasmodium vivax populations in the province were examined. Forty-five P. falciparum and 67 P. vivax samples collected in the 2008 baseline survey were successfully genotyped using eight P. falciparum and seven P. vivax microsatellite markers. Genetic diversity, relationships and distribution of both P. falciparum and P. vivax populations were analysed. Plasmodium falciparum population exhibited low diversity with 19 haplotypes identified and had closely related clusters indicating clonal expansion. Interestingly, a dominant haplotype was significantly associated with fever and high parasite density. In contrast, the P. vivax population was highly diverse with 58 haplotypes identified that were not closely related. Parasite populations between different islands in the province showed low genetic differentiation. The low diversity and clonal population of P. falciparum population may partially account for clinical immunity developed against illness. However, it is possible that importation of a new P. falciparum strain was the major cause of illness. High diversity in P. vivax population and low relatedness between strains suggested clinical immunity to P. vivax may be maintained by different mechanisms. The genetic diversity, population structure and distribution of strains indicate that transmission of P. falciparum was low, but that of P. vivax was still high in 2008. These data will be useful for assessing changes in malaria transmission resulting from interventions.
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DOI：
10.4269/ajtmh.1996.54.325
发表时间：
1996-04-01
影响因子：
3.3
作者：
Roper, C;Elhassan, IM;Arnot, DE
通讯作者：
Arnot, DE
DOI：
10.1590/s0074-02762008000700007
发表时间：
2008-11-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Cucunubá, Zulma Milena;Guerra, Ángela Patricia;Nicholls, Rubén Santiago
通讯作者：
Nicholls, Rubén Santiago
影响因子：
3.2
作者：
Feil, EJ;Li, BC;Spratt, BG
通讯作者：
Spratt, BG
影响因子：
3
作者：
Alves, Joana;Roque, Ana Luisa;Arez, Ana Paula
通讯作者：
Arez, Ana Paula
影响因子：
3
作者：
Francisco AP;Vaz C;Monteiro PT;Melo-Cristino J;Ramirez M;Carriço JA
通讯作者：
Carriço JA