Low iron availability in continuous in vitro colonic fermentations induces strong dysbiosis of the child gut microbial consortium and a decrease in main metabolites.
Low iron availability in continuous in vitro colonic fermentations induces strong dysbiosis of the child gut microbial consortium and a decrease in main metabolites.
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连续的体外结肠发酵中铁的低可用性会诱导儿童肠道微生物联盟的强大营养不良，而主代谢产物的降低。
DOI：
10.1111/j.1574-6941.2012.01461.x
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发表时间：
2013-01
影响因子：
4.2
通讯作者：
Lacroix C
中科院分区：
文献类型：
--
作者：
Dostal A;Fehlbaum S;Chassard C;Zimmermann MB;Lacroix C
据估计，全世界有20亿人受到铁缺乏的影响，补充铁是一种常见的纠正策略。采用固定化粪便微生物群体外发酵模型，研究了缺铁和补铁对儿童肠道复合微生物群落的影响。模拟缺铁和补铁的模拟缺铁和补铁的乳糜液(全部Fe经2，2‘-联吡啶螯合至26.5mgFe L-1)连续发酵。采用定量聚合酶链式反应、16S rRNA基因454焦磷酸测序和高效液相色谱对发酵液样品进行微生物组成和代谢产物分析。低铁条件下(1.56mgFe L~(-1))显著降低醋酸盐浓度，随后补充铁(26.5mgFe L~(-1))可恢复醋酸盐产量。正常铁条件下的高铁对肠道微生物区系组成和代谢活性没有影响。在非常低的铁条件下(0。9mg F e L-1或2，2‘-联吡啶螯合的铁)，减少了玫瑰草属/直肠真细菌属、簇状梭状芽胞杆菌IV成员和类杆菌属的数量。乳杆菌属(Lactobacillusspp.)肠杆菌科细菌的增加与丁酸盐(-84%)和丙酸(-55%)的下降一致。在极低铁的条件下，肠道微生物区系的严重失调以及主要肠道微生物区系代谢物的减少可能会削弱微生物区系的屏障效应，并对肠道健康造成负面影响。
Iron (Fe) deficiency affects an estimated 2 billion people worldwide and Fe supplements are a common corrective strategy. The impact of Fe deficiency and Fe supplementation on the complex microbial community of the child gut was studied using in vitro colonic fermentation models inoculated with immobilized fecal microbiota. Chyme media (all Fe chelated by 2,2’-dipyridyl to 26.5 mg Fe L-1) mimicking Fe deficiency and supplementation were continuously fermented. Fermentation effluent samples were analyzed daily on the microbial composition and metabolites by qPCR, 16S rRNA gene 454-pyrosequencing and HPLC. Low Fe conditions (1.56 mg Fe L-1) significantly decreased acetate concentrations and subsequent Fe supplementation (26.5 mg Fe L-1) restored acetate production. High Fe following normal Fe conditions had no impact on the gut microbiota composition and metabolic activity. During very low Fe conditions (0 . 9 m g F e L-1 or Fe chelated b y 2,2’-dipyridyl), a decrease of Roseburia spp./Eubacterium rectale, Clostridium Cluster IV members and Bacteroides spp. was observed while Lactobacillus spp. and Enterobacteriaceae increased consistent with a decrease of butyrate (-84%) and propionate (-55%). The strong dysbiosis of the gut microbiota together with decrease of main gut microbiota metabolites observed with very low iron conditions could weaken the barrier effect of the microbiota and negatively impact gut health.
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影响因子：
1.9
作者：
HOVE, H;NORDGAARDANDERSEN, I;MORTENSEN, PB
通讯作者：
MORTENSEN, PB
影响因子：
64.8
作者：
Fukuda, Shinji;Toh, Hidehiro;Ohno, Hiroshi
通讯作者：
Ohno, Hiroshi
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars
影响因子：
4
作者：
Cleusix, V.;Lacroix, C.;Le Blay, G.
通讯作者：
Le Blay, G.
影响因子：
3.6
作者：
Balamurugan, Ramadass;Mary, R. Regina;Ramakrishna, Balakrishnan S.
通讯作者：
Ramakrishna, Balakrishnan S.