Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus.
Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus.
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Bxy-laf-1对松材线虫繁殖行为的调控作用
DOI：
10.3389/fphys.2022.1024409
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发表时间：
2022
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
松材线虫是一种世界性的检疫性线虫，给许多国家造成了巨大的经济损失和生态灾难。B的性比。松材线虫在线虫侵染中起重要作用。laf-1相关基因在动物中高度保守，在性别决定中起着重要作用。因此，我们研究了其直向同源物Bxy-laf-1在B中的表达模式和生物学功能。木生植物Bxy-laf-1具有两个典型的保守的DNA结合结构域DEAD和Helicase C。实时定量PCR数据显示，Bxy-laf-1的表达需要在整个生命的B。在J2期表达量最高，成虫期表达量最低。mRNA原位杂交结果表明，Bxy-laf-1主要定位于B的头咽部和生殖器官。木生植物RNA干扰（RNAi）表明，头部摆动频率显着降低。RNA干扰的结果显示，在孵化的幼虫中观察到运动的显着降低。F_0和F_1代的雌雄性比也有所下降，但F_2代有所恢复。雌成虫的尾部出现畸形。这种现象在F0和F1代出现，但在F2代恢复。Bxy-laf-1是一个典型的性别决定基因，在雄性和雌性中具有不同的表达模式。正如在其他物种中所证明的那样，在敲低Bxy-laf-1表达后，性别比发生了改变。本研究结果有助于我们理解Bxy-laf-1在B中的分子过程。这可能为如何通过抑制个体发育和降低线虫繁殖力来控制松材线虫病提供线索。
Bursaphelenchus xylophilu is a worldwide quarantine nematode, causing huge economic losses and ecological disasters in many countries. The sex ratio of B. xylophilus plays an important role in the nematode infestation. The laf-1-related genes are highly conserved in animals, playing crucial roles in sex determination. Therefore, we investigated the expression pattern and biological function of its orthologue, Bxy-laf-1 in B. xylophilus. Bxy-laf-1 has two typical conserved DNA-binding domains, DEAD and Helicase C. The real-time quantitative PCR data revealed that Bxy-laf-1 expression was required throughout the entire life of B. xylophilus, with the maximum expression in the J2 stage and the lowest expression in the adult stage. mRNA in situ hybridization showed that Bxy-laf-1 is mainly located in the cephalopharynx and reproductive organs of B. xylophilus. RNA interference (RNAi) indicated that the head swing frequency was dramatically decreased. The RNA interference results displayed that a significant reduction in motility was observed in the hatched larvae. The female to male sex ratio was also decreased in the F0 and F1 generations, but recovered in the F2 generation. The tail of female adults with eggs in the belly appeared deformities. This phenomenon appeared in the F0 and F1 generations, but recovered in the F2 generation. Bxy-laf-1 is a typical sex-determination gene with distinct expression patterns in males and females. As demonstrated in other species, the sex ratio was altered after knocking down Bxy-laf-1 expression. The results of this study contribute to our understanding of the molecular processes of Bxy-laf-1 in B. xylophilus, which may provide clues about how to control pine wilt disease by inhibiting ontogenic growth and reducing nematode fertility.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
6.7
作者：
Kikuchi T;Cotton JA;Dalzell JJ;Hasegawa K;Kanzaki N;McVeigh P;Takanashi T;Tsai IJ;Assefa SA;Cock PJ;Otto TD;Hunt M;Reid AJ;Sanchez-Flores A;Tsuchihara K;Yokoi T;Larsson MC;Miwa J;Maule AG;Sahashi N;Jones JT;Berriman M
通讯作者：
Berriman M
影响因子：
64.5
作者：
HAY, B;JAN, LY;JAN, YN
通讯作者：
JAN, YN
影响因子：
3
作者：
Cui, Jing;Li, Yong-Xia;Zhang, Xing-Yao
通讯作者：
Zhang, Xing-Yao
影响因子：
64.8
作者：
LASKO, PF;ASHBURNER, M
通讯作者：
ASHBURNER, M