Portable microfluidic integrated plasmonic platform for pathogen detection.
Portable microfluidic integrated plasmonic platform for pathogen detection.
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DOI：
10.1038/srep09152
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发表时间：
2015-03-24
影响因子：
4.6
通讯作者：
Demirci U
中科院分区：
文献类型：
--
作者：
Tokel O;Yildiz UH;Inci F;Durmus NG;Ekiz OO;Turker B;Cetin C;Rao S;Sridhar K;Natarajan N;Shafiee H;Dana A;Demirci U
及时检测病原体是传染病早期诊断和治疗的关键。传统的病原体检测方法，例如酶联免疫吸附测定（ELISA）、培养或聚合酶链反应（PCR）需要长的测定时间以及复杂和昂贵的仪器，这些方法不适合资源受限以及初级保健环境下的护理点（POC）需要。因此，存在开发用于在POC检测病原体的简单、快速和准确的方法的未满足的需求。在此，我们提出了一种便携式、多路、廉价的微流体集成表面等离子体共振（SPR）平台，其检测和定量细菌，即，大肠杆菌（E. Coli)和金黄色葡萄球菌(S.金黄色）。该平台实现了对E.在磷酸盐缓冲盐水（PBS）和腹膜透析（PD）液中，浓度范围为~105至3.2 × 107 CFU/mL的大肠杆菌。同时，对该平台的多重性和特异性进行了检测。金黄色葡萄球菌样品。所提出的平台技术可能适用于在POC和初级保健环境中捕获和检测其他病原体。
Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid, and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e., Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ~105 to 3.2 × 107 CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings.
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影响因子：
7.4
作者：
Maalouf, Rita;Fournier-Wirth, Chantal;Jaffrezic-Renault, Nicole
通讯作者：
Jaffrezic-Renault, Nicole
影响因子：
4
作者：
Lissandrello, C.;Inci, F.;Ekinci, K. L.
通讯作者：
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影响因子：
6.1
作者：
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通讯作者：
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影响因子：
16.1
作者：
Lee, Won Gu;Kim, Yun-Gon;Chung, Bong Geun;Demirci, Utkan;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
2.8
作者：
Huang, Chengjun;Bonroy, Kristien;Borghs, Gustaaf
通讯作者：
Borghs, Gustaaf