Necrosis-induced apoptosis promotes regeneration in Drosophila wing imaginal discs.
Necrosis-induced apoptosis promotes regeneration in Drosophila wing imaginal discs.
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DOI：
10.1093/genetics/iyab144
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发表时间：
2021-11-05
期刊：
影响因子：
3.3
通讯作者：
Harris RE
中科院分区：
文献类型：
--
作者：
Klemm J;Stinchfield MJ;Harris RE
再生是一个复杂的过程，需要对组织损失做出协调的遗传反应。来自垂死细胞的信号对于这一过程至关重要，并且在程序性细胞死亡（如细胞凋亡）后的再生背景下可以得到最好的理解。相反，不受控制的死亡形式（例如坏死）后的再生尚未得到充分探索。在这里，我们开发了一种使用果蝇翼成虫盘研究坏死后再生的方法。我们发现坏死刺激再生的水平与细胞凋亡介导的细胞死亡相当，并在涉及局部 JNK 信号传导的伤口边缘激活类似的反应。然而，出乎意料的是，坏死也会导致远离消融部位的显着细胞凋亡，我们将其称为坏死诱导的细胞凋亡（NiA）。这种细胞凋亡的发生独立于伤口边缘的变化，重要的是不依赖于 JNK 信号传导。此外，我们发现阻断 NiA 会限制增殖并随后抑制再生，这表明坏死受损的组织可以在远离损伤的地方激活程序性细胞死亡，从而促进再生。
Regeneration is a complex process that requires a coordinated genetic response to tissue loss. Signals from dying cells are crucial to this process and are best understood in the context of regeneration following programmed cell death, like apoptosis. Conversely, regeneration following unregulated forms of death, such as necrosis, have yet to be fully explored. Here, we have developed a method to investigate regeneration following necrosis using the Drosophila wing imaginal disc. We show that necrosis stimulates regeneration at an equivalent level to that of apoptosis-mediated cell death and activates a similar response at the wound edge involving localized JNK signaling. Unexpectedly, however, necrosis also results in significant apoptosis far from the site of ablation, which we have termed necrosis-induced apoptosis (NiA). This apoptosis occurs independent of changes at the wound edge and importantly does not rely on JNK signaling. Furthermore, we find that blocking NiA limits proliferation and subsequently inhibits regeneration, suggesting that tissues damaged by necrosis can activate programmed cell death at a distance from the injury to promote regeneration.
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影响因子：
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作者：
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作者：
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DOI：
10.1038/nrm3999
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通讯作者：
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