Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice.
Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice.
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DOI：
10.3791/63344
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发表时间：
2022-01-21
影响因子：
1.2
通讯作者：
VanderHorst, Veronique
中科院分区：
文献类型：
--
作者：
Joshi, Krutika;Kirby, Alana;Niu, Jianguo;VanderHorst, Veronique
针对小鼠脑部位的立体定位手术通常由颅骨标志引导。然后通过颅骨钻孔进入。由于特定的解剖学挑战，这种标准方法对于尾侧脑干和上颈髓中的目标可能具有挑战性，因为这些部位远离颅骨标志，导致不精确。在这里，我们概述了一种替代的立体定向方法，通过枕大池，已被用来针对离散地区的利益，在尾侧脑干和上颈髓。枕大池从枕骨延伸到寰椎（即，第二椎骨）充满脑脊液，并被硬脑膜覆盖。这种方法提供了一种可重复的进入途径，以选择由于解剖屏障而难以到达的中枢神经系统（CNS）结构。此外，它允许直接可视化靠近目标部位的脑干标志，在向尾侧脑干和上颈髓中的受限感兴趣区域输送小注射体积时提高准确性。最后，这种方法避免了对运动和感觉运动研究很重要的小脑。在小鼠中靶向脑部位的立体定位手术通常涉及通过颅骨进入并由颅骨标志引导。在这里，我们概述了一种替代的立体定位方法，通过枕大池，依赖于脑干标志的直接可视化，以靶向尾侧脑干和上颈段脊髓。
Stereotaxic surgery to target brain sites in mice is commonly guided by skull landmarks. Access is then obtained via burr holes drilled through the skull. This standard approach can be challenging for targets in the caudal brainstem and upper cervical cord due to specific anatomical challenges as these sites are remote from skull landmarks, leading to imprecision. Here we outline an alternative stereotaxic approach via the cisterna magna that has been used to target discrete regions of interest in the caudal brainstem and upper cervical cord. The cisterna magna extends from the occipital bone to the atlas (i.e., the second vertebral bone), is filled with cerebrospinal fluid, and is covered by dura mater. This approach provides a reproducible route of access to select central nervous system (CNS) structures that are otherwise hard to reach due to anatomical barriers. Furthermore, it allows for direct visualization of brainstem landmarks in close proximity to target sites, increasing accuracy when delivering small injection volumes to restricted regions of interest in the caudal brainstem and upper cervical cord. Finally, this approach avoids the cerebellum which can be important for motor and sensorimotor studies. Stereotaxic surgery to target brain sites in mice commonly involves access through the skull bones and is guided by skull landmarks. Here we outline an alternative stereotaxic approach to target the caudal brainstem and upper cervical spinal cord via the cisterna magna that relies on direct visualization of brainstem landmarks.
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影响因子：
3
作者：
Blasiak, Tomasz;Czubak, Wojciech;Lewandowski, Marian H.
通讯作者：
Lewandowski, Marian H.
影响因子：
4.6
作者：
Rangarajan JR;Vande Velde G;van Gent F;De Vloo P;Dresselaers T;Depypere M;van Kuyck K;Nuttin B;Himmelreich U;Maes F
通讯作者：
Maes F
C57BL/6J小鼠中孤立道的核：亚核拟层，鼓膜神经投影和脑干连接。
影响因子：
2.5
作者：
Ganchrow, Donald;Ganchrow, Judith R.;Cicchini, Vanessa;Bartel, Dianna L.;Kaufman, Daniel;Girard, David;Whitehead, Mark C.
通讯作者：
Whitehead, Mark C.
DOI：
10.3791/50004
发表时间：
2012-10-29
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Ung K;Arenkiel BR
通讯作者：
Arenkiel BR
影响因子：
15.9
作者：
Krashes, Michael J.;Koda, Shuichi;Lowell, Bradford B.
通讯作者：
Lowell, Bradford B.