Image-based in vivo assessment of targeting accuracy of stereotactic brain surgery in experimental rodent models.
Image-based in vivo assessment of targeting accuracy of stereotactic brain surgery in experimental rodent models.
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DOI：
10.1038/srep38058
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发表时间：
2016-11-30
影响因子：
4.6
通讯作者：
Maes F
中科院分区：
文献类型：
--
作者：
Rangarajan JR;Vande Velde G;van Gent F;De Vloo P;Dresselaers T;Depypere M;van Kuyck K;Nuttin B;Himmelreich U;Maes F
立体定向神经外科用于实验性大鼠和小鼠模型中神经和精神疾病的临床前研究，将针或电极植入大脑中的预定位置。然而，不准确的靶向可能会混淆此类实验的结果。与临床实践相反，啮齿类动物的不准确靶向通常不会被注意到，直到通过离体终点组织学进行评估。我们在这里提出了一种基于多模态术后成像的小动物研究中立体定向准确性体内评估的工作流程。每只动物的手术轨迹都是根据术后 CT 成像的物理植入物和/或术后 MRI 中可见的痕迹以 3D 方式重建的。通过将个体动物的术后图像共同配准到通用立体定位模板，可以量化目标准确性。两个常用的神经调节区域被用作目标。目标定位错误不仅表现出可变性，而且表现出瞄准的不准确性。只有约 30% 的电极位于目标亚核结构内，并且还注意到了特定的副作用。从侵入性/主观 2D 组织学转向基于客观体内 3D 成像的靶向精度评估可能会通过在研究早期排除脱靶病例而有利于更有效地利用实验数据。
Stereotactic neurosurgery is used in pre-clinical research of neurological and psychiatric disorders in experimental rat and mouse models to engraft a needle or electrode at a pre-defined location in the brain. However, inaccurate targeting may confound the results of such experiments. In contrast to the clinical practice, inaccurate targeting in rodents remains usually unnoticed until assessed by ex vivo end-point histology. We here propose a workflow for in vivo assessment of stereotactic targeting accuracy in small animal studies based on multi-modal post-operative imaging. The surgical trajectory in each individual animal is reconstructed in 3D from the physical implant imaged in post-operative CT and/or its trace as visible in post-operative MRI. By co-registering post-operative images of individual animals to a common stereotaxic template, targeting accuracy is quantified. Two commonly used neuromodulation regions were used as targets. Target localization errors showed not only variability, but also inaccuracy in targeting. Only about 30% of electrodes were within the subnucleus structure that was targeted and a-specific adverse effects were also noted. Shifting from invasive/subjective 2D histology towards objective in vivo 3D imaging-based assessment of targeting accuracy may benefit a more effective use of the experimental data by excluding off-target cases early in the study.
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影响因子：
3
作者：
Blasiak, Tomasz;Czubak, Wojciech;Lewandowski, Marian H.
通讯作者：
Lewandowski, Marian H.
影响因子：
5.7
作者：
Dorr, A.;Sled, J. G.;Kabani, N.
通讯作者：
Kabani, N.
影响因子：
4
作者：
Paralikar, K. J.;Neuberger, T.;Clement, R. S.
通讯作者：
Clement, R. S.
影响因子：
3
作者：
Fung, SH;Burstein, D;Born, RT
通讯作者：
Born, RT
影响因子：
4.1
作者：
Nowinski, Wieslaw L.;Chua, Beng Choon;Knopp, Michael V.
通讯作者：
Knopp, Michael V.