The effect of fiber alignment and heparin coating on cell infiltration into nanofibrous PLLA scaffolds.
The effect of fiber alignment and heparin coating on cell infiltration into nanofibrous PLLA scaffolds.
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DOI：
10.1016/j.biomaterials.2010.01.062
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发表时间：
2010-05
期刊：
影响因子：
14
通讯作者：
Li, Song
中科院分区：
文献类型：
--
作者：
Kurpinski, Kyle T.;Stephenson, Jacob T.;Janairo, Randall Raphael R.;Lee, Hanmin;Li, Song
可生物降解纳米纤维模拟了天然细胞外基质的纤维结构，为组织再生提供了细胞友好的微环境。然而，纳米纤维组织和固定化生化因子对细胞浸润三维支架的影响尚不清楚。例如，由于纤维之间相对较小的孔径，细胞渗入电纺纳米纤维基质往往受到限制。在体外和体内实验中，我们发现纳米纤维支架的生物物理和生化修饰促进了内皮细胞在三维支架中的浸润。在体外实验中，排列整齐的纳米纤维显著增强了细胞对纳米纤维基质的浸润。在全层真皮创面模型中，与没有支架的对照组相比，纳米纤维支架增强了表皮皮肤细胞在创面上的迁移。排列整齐的纳米纤维促进内皮细胞向支架的浸润。此外，肝素包被纳米纤维也显著增加细胞浸润。这些结果揭示了纳米纤维的生物物理和生化特性在调节细胞浸润到三维支架和组织重塑中的重要性。
Biodegradable nanofibers simulate the fibril structure of natural extracellular matrix, and provide a cell-friendly microenvironment for tissue regeneration. However, the effects of nanofiber organization and immobilized biochemical factors on cell infiltration into three-dimensional scaffolds are not well understood. For example, cell infiltration into an electrospun nanofibrous matrix is often limited due to relatively small pore size between the fibers. Here we showed that biophysical and biochemical modification of nanofibrous scaffolds facilitated endothelial cell infiltration in three-dimensional scaffolds in vitro and in vivo. Aligned nanofibers significantly enhanced cell infiltration into the nanofibrous matrices in vitro. In a full-thickness dermal wound model, the nanofiber scaffolds enhanced epidermal skin cell migration across the wound when compared to a control group without scaffold. Aligned nanofibers promoted the infiltration of endothelial cells into the scaffolds. Furthermore, heparin-coated nanofibers also increased cell infiltration significantly. These results shed light on the importance of biophysical and biochemical properties of nanofibers in the regulation of cell infiltration into three-dimensional scaffolds and tissue remodeling.
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影响因子：
3.1
作者：
Boland, ED;Matthews, JA;Bowlin, GL
通讯作者：
Bowlin, GL
DOI：
10.1073/pnas.0704581104
发表时间：
2007-07-17
影响因子：
11.1
作者：
Hashi, Craig K.;Zhu, Yiqian;Li, Song
通讯作者：
Li, Song
影响因子：
3.9
作者：
Metcalfe, AD;Ferguson, MWJ
通讯作者：
Ferguson, MWJ
影响因子：
4.6
作者：
Zong, XH;Ran, SF;Chu, B
通讯作者：
Chu, B
影响因子：
--
作者：
Ma, ZW;Kotaki, M;Ramakrishna, S
通讯作者：
Ramakrishna, S