Monitoring in Real Time the Formation and Removal of Biofilms from Clinical Related Pathogens Using an Impedance-Based Technology.
Monitoring in Real Time the Formation and Removal of Biofilms from Clinical Related Pathogens Using an Impedance-Based Technology.
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DOI：
10.1371/journal.pone.0163966
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Ruas-Madiedo P
中科院分区：
文献类型：
--
作者：
Gutiérrez D;Hidalgo-Cantabrana C;Rodríguez A;García P;Ruas-Madiedo P
在不同的生态系统中发现的细菌生长在一个有利于它们生存和定居的聚集细胞群落中。不同的细胞外聚合物被用来捕获这种多物种群落，形成生物膜，它可以与生物和非生物表面。然而，这种广泛而成功的细菌生活方式可能会对人类活动产生负面影响，因为许多病原体和腐败细菌形成不易根除的生物膜。因此，寻找新的抗生物膜生物活性分子是一个非常活跃的研究领域，需要简单，可靠，快速的筛选方法。在这项工作中，我们已经成功地验证了一个阻抗为基础的方法，最初开发的研究粘附真核细胞，监测形成的单种生物膜的三种模式细菌在真实的时间。xCelligence真实的时间细胞分析仪（RTCA）设备使用涂有金微电极的特定微量滴定E板，其检测粘附细胞的附着，从而修改阻抗信号。在目前的研究中，该技术允许区分金黄色葡萄球菌和表皮葡萄球菌的生物膜生产者和非生产者，以及仅当培养基中存在蔗糖时变形链球菌生物膜的形成。此外，不同的阻抗值允许的歧视之间的生物膜生产菌株的测试，无论聚合物生物膜基质的性质。最后，我们连续监测了噬菌体phi-IPLA 7和噬菌体编码的内溶素LysH 5对葡萄球菌生物膜形成的抑制，以及通过最后的抗微生物处理去除预先形成的生物膜。与阻抗为基础的方法观察到的结果显示出高度的相关性与标准的方法，如结晶紫染色和细菌计数，以及与其他非生物表面（聚苯乙烯和不锈钢）上获得的。因此，这种RTCA技术在生物膜研究竞技场中开辟了新的机会，并且可以进一步探索其在其他细菌属以及不同生物活性分子中的应用。
Bacteria found in diverse ecosystems grow in a community of aggregated cells that favors their survival and colonization. Different extracellular polymeric substances are used to entrap this multispecies community forming a biofilm, which can be associated to biotic and abiotic surfaces. This widespread and successful way of bacterial life, however, can lead to negative effects for human activity since many pathogen and spoiling bacteria form biofilms which are not easy to eradicate. Therefore, the search for novel anti-biofilm bio-active molecules is a very active research area for which simple, reliable, and fast screening methods are demanded. In this work we have successfully validated an impedance-based method, initially developed for the study of adherent eukaryotic cells, to monitor the formation of single-species biofilms of three model bacteria in real time. The xCelligence real time cell analyzer (RTCA) equipment uses specific microtiter E-plates coated with gold-microelectrodes that detect the attachment of adherent cells, thus modifying the impedance signal. In the current study, this technology allowed the distinction between biofilm-producers and non-producers of Staphylococcus aureus and Staphylococcus epidermidis, as well as the formation of Streptococcus mutans biofilms only when sucrose was present in the culture medium. Besides, different impedance values permitted discrimination among the biofilm-producing strains tested regardless of the nature of the polymeric biofilm matrix. Finally, we have continuously monitored the inhibition of staphylococcal biofilm formation by the bacteriophage phi-IPLA7 and the bacteriophage-encoded endolysin LysH5, as well as the removal of a preformed biofilm by this last antimicrobial treatment. Results observed with the impedance-based method showed high correlation with those obtained with standard approaches, such as crystal violet staining and bacteria enumeration, as well as with those obtained upon other abiotic surfaces (polystyrene and stainless steel). Therefore, this RTCA technology opens new opportunities in the biofilm research arena and its application could be further explored for other bacterial genera as well as for different bio-active molecules.
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影响因子：
5.6
作者：
Cihalova K;Chudobova D;Michalek P;Moulick A;Guran R;Kopel P;Adam V;Kizek R
通讯作者：
Kizek R
影响因子：
2.4
作者：
Kelly, D.;McAuliffe, O.;Coffey, A.
通讯作者：
Coffey, A.
影响因子：
4.2
作者：
Jeon JG;Rosalen PL;Falsetta ML;Koo H
通讯作者：
Koo H
影响因子：
3.2
作者：
Koo, H.;Xiao, J.;Jeon, J. G.
通讯作者：
Jeon, J. G.
影响因子：
3.4
作者：
Fenton M;Keary R;McAuliffe O;Ross RP;O'Mahony J;Coffey A
通讯作者：
Coffey A