Circadian Clock Gene Period Contributes to Diapause via GABAeric-Diapause Hormone Pathway in Bombyx mori.
Circadian Clock Gene Period Contributes to Diapause via GABAeric-Diapause Hormone Pathway in Bombyx mori.
复制标题
生物钟基因周期通过 GABAeric-滞育激素途径促进家蚕滞育
DOI：
10.3390/biology10090842
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发表时间：
2021-08-30
期刊：
影响因子：
4.2
通讯作者：
Xu SQ
中科院分区：
文献类型：
--
作者：
Cui WZ;Qiu JF;Dai TM;Chen Z;Li JL;Liu K;Wang YJ;Sima YH;Xu SQ
昆虫滞育是生物钟和内分泌系统共同调控的一个经典而长期关注的课题。许多昆虫的研究表明，生物钟系统的紊乱会影响滞育的发生。然而，生物钟与内分泌激素调控滞育发生的具体分子调控机制和关键节点尚缺乏有见地的报道。本研究以家蚕周期基因敲除突变系的滞育变化为基础，确定了家蚕生物钟转录-翻译反馈环调控滞育激素水平和作用的分子节点和途径。本研究证实了家蚕周期基因敲除改变了经典的温度和光照依赖性滞育的命运，并通过GABA-DH神经递质-内分泌激素途径改变滞育，表明GABA受体基因GRD受家蚕生物钟和内分泌系统的共同控制。研究结果为解释家蚕生物钟对内分泌激素的调控机制提供了一个实例。摘要滞育是昆虫在季节性逆境适应的基础上发生的一种发育转变，受生物钟系统和内分泌系统的调节。然而，这些系统的作用的分子节点及其机制仍然不清楚。在这里，构建了一个突变体的昼夜节律钟基因周期（Per）敲除，这大大改变了经典的滞育注定的途径。全基因敲除的家蚕强烈减弱，但不能完全阻断，温度和光周期对滞育决定的预定影响，这种影响依赖于滞育激素（DH）途径。缺乏Per基因的生物钟系统的转录-翻译反馈环受损，导致γ-氨基丁酸（GABA）受体GRD的表达通过改变Cycle的表达水平而直接上调。脑-食管下神经节组织复合体中GABA的合成继而增加并限制其分解，从而不断促进GABA能信号发挥作用，最终抑制（延迟）DH向血淋巴的释放，降低DH的滞育诱导作用。研究结果为解释家蚕生物钟对内分泌激素的调控机制提供了一个实例。
Simple Summary Diapause in insects is a classic and long-term concern subject regulated by both circadian clock and endocrine system. Studies in many insects have shown that disturbance of circadian clock system can affect diapause occurrence. However, the specific molecular regulation mechanism and key nodes between circadian clock and endocrine hormones regulating diapause occurrence are still lack of insightful reports. Our work identified the molecular nodes and pathways through which the transcription-translation feedback loop of the silkworm circadian clock regulated the level and action of diapause hormones, based on the diapause change in a silkworm mutant line of Period gene knockout. This work confirmed that Period knocked out in silkworms changed the classic temperature- and photoperiodic-dependent diapause-destiny and changed the diapause through the GABA-DH neurotransmitter-endocrine hormone pathway, and showed that the GABA receptor gene, GRD, was controlled by both the circadian clock and endocrine system in silkworms. The results provided an example to explain the regulatory mechanism of the circadian clock on endocrine hormones in the silkworm. Abstract Diapause is a developmental transition in insects based on seasonal adaptation to adversity; it is regulated by a circadian clock system and the endocrine system. However, the molecular node and its mechanism underlying the effects of these systems are still unclear. Here, a mutant of Bombyx mori with the circadian clock gene Period (Per) knocked out was constructed, which dramatically changed the classic diapause-destined pathway. Per-knockout silkworms powerfully attenuated, but could not completely block, the predetermined effects of temperature and photoperiod on diapause determination, and this effect depended on the diapause hormone (DH) pathway. The impaired transcription-translation feedback loop of the circadian clock system lacking the Per gene caused direct up-regulation of the expression of GRD, a receptor of γ-aminobutyric acid (GABA), by changing expression level of Cycle. The synthesis of GABA in the tissue complex of brain-suboesophageal ganglion then increased and restricted the decomposition, which continuously promoted the GABAergic signal to play a role, and finally inhibiting (delaying) the release of DH to the hemolymph, and reducing the diapause-inducing effect of DH. The results provided an example to explain the regulatory mechanism of the circadian clock on endocrine hormones in the silkworm.
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影响因子：
16.6
作者：
Barca-Mayo O;Pons-Espinal M;Follert P;Armirotti A;Berdondini L;De Pietri Tonelli D
通讯作者：
De Pietri Tonelli D
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