Neutrophils enhance early Trypanosoma brucei infection onset.
Neutrophils enhance early Trypanosoma brucei infection onset.
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DOI：
10.1038/s41598-018-29527-y
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发表时间：
2018-07-25
影响因子：
4.6
通讯作者：
Van Den Abbeele J
中科院分区：
文献类型：
--
作者：
Caljon G;Mabille D;Stijlemans B;De Trez C;Mazzone M;Tacchini-Cottier F;Malissen M;Van Ginderachter JA;Magez S;De Baetselier P;Van Den Abbeele J
在这项研究中，布氏锥虫通过感染的Glossina morsitans采采蝇的叮咬自然传播给小鼠。中性粒细胞被迅速招募到叮咬部位，而单核细胞则被逐渐吸引。在寄生虫接种部位，炎性细胞因子（il 1b，il 6），il 10和中性粒细胞趋化因子（cxcl 1，cxcl 5）的表达瞬时上调。然后，发生第二次中性粒细胞流入，与先前描述的寄生虫在耳真皮中的滞留和扩张相吻合。先天性和实验性中性粒细胞减少症模型，结合生物发光成像，表明中性粒细胞没有显着贡献的皮肤寄生虫控制和感染发作期间引起更高的全身寄生虫血症水平。很少观察到皮肤中性粒细胞吞噬寄生虫，仅限于运动性/活力降低的寄生虫，而活寄生虫逃避吞噬作用。据我们所知，这项研究首次描述了锥虫感染对感染性舌蝇叮咬后早期先天免疫反应的促进作用。我们的数据表明，锥虫在其早期发育中没有受到阻碍，并且受益于宿主的先天性反应，其中嗜中性粒细胞是早期感染的重要调节因子，正如已经证明的白蛉传播的利什曼原虫寄生虫。
In this study, Trypanosoma brucei was naturally transmitted to mice through the bites of infected Glossina morsitans tsetse flies. Neutrophils were recruited rapidly to the bite site, whereas monocytes were attracted more gradually. Expression of inflammatory cytokines (il1b, il6), il10 and neutrophil chemokines (cxcl1, cxcl5) was transiently up-regulated at the site of parasite inoculation. Then, a second influx of neutrophils occurred that coincided with the previously described parasite retention and expansion in the ear dermis. Congenital and experimental neutropenia models, combined with bioluminescent imaging, indicate that neutrophils do not significantly contribute to dermal parasite control and elicit higher systemic parasitemia levels during the infection onset. Engulfment of parasites by neutrophils in the skin was rarely observed and was restricted to parasites with reduced motility/viability, whereas live parasites escaped phagocytosis. To our knowledge, this study represents the first description of a trypanosome infection promoting role of early innate immunological reactions following an infective tsetse fly bite. Our data indicate that the trypanosome is not hindered in its early development and benefits from the host innate responses with the neutrophils being important regulators of the early infection, as already demonstrated for the sand fly transmitted Leishmania parasite.
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影响因子：
6.7
作者：
Hurrell BP;Schuster S;Grün E;Coutaz M;Williams RA;Held W;Malissen B;Malissen M;Yousefi S;Simon HU;Müller AJ;Tacchini-Cottier F
通讯作者：
Tacchini-Cottier F
影响因子：
2.4
作者：
BARRY, JD;EMERY, DL
通讯作者：
EMERY, DL
影响因子：
3.9
作者：
Bai, Xuewei;Yao, Huimin;Rong, Mingqiang
通讯作者：
Rong, Mingqiang
影响因子：
3.1
作者：
Caljon, Guy;Van Den Abbeele, Jan;Magez, Stefan
通讯作者：
Magez, Stefan
影响因子：
7.3
作者：
Regli IB;Passelli K;Hurrell BP;Tacchini-Cottier F
通讯作者：
Tacchini-Cottier F