Genome-Wide Characterization of DNA Demethylase Genes and Their Association with Salt Response in Pyrus.
Genome-Wide Characterization of DNA Demethylase Genes and Their Association with Salt Response in Pyrus.
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DNA 去甲基化酶基因的全基因组表征及其与梨属盐响应的关联
DOI：
10.3390/genes9080398
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发表时间：
2018-08-06
期刊：
影响因子：
3.5
通讯作者：
Chang Y
中科院分区：
文献类型：
--
作者：
Liu C;Li H;Lin J;Wang Y;Xu X;Cheng ZM;Chang Y
DNA甲基化在基因组保护和基因表达调控中起着重要作用，与植物对环境的反应有关。DNA去甲基化酶是DNA甲基化调控中非常重要的蛋白质。在这项研究中，我们对梨中假定的去甲基化酶（DMEs）进行了全基因组和深度分析。在梨基因组中发现了7个DME基因，根据它们的结构域组织将它们定义为PbDME1-7。结果得到了基因结构特征和系统发育分析的支持。DME基因结构相对复杂，DME7蛋白不含Perm_CXXC结构域。DME基因在梨和苹果的祖先基因组中经历了一次全基因组重复事件（WGD），该事件发生在梨和苹果的k值分化之前。表达结果表明，高盐胁迫会影响白桦树DMEs和盐响应基因的表达水平。此外，盐胁迫处理改变了盐应答基因的甲基化水平。结果提示PbDME基因在盐胁迫响应中发挥重要作用，有助于更好地了解该基因的复杂功能，为梨树耐盐表观遗传学研究提供依据。
DNA methylation plays important roles in genome protection and the regulation of gene expression and it is associated with plants’ responses to environments. DNA demethylases are very important proteins in DNA methylation regulation. In this study, we performed genome-wide and deep analysis of putative demethylases (DMEs) in pear. Seven DME genes were found in the pear genome and were defined as PbDME1–7 based on their domain organization. Results were supported by the gene structural characteristics and phylogenetic analysis. The gene structure of the DME genes were relatively complex and the DME7 proteins didn’t contain the Perm_CXXC domain. The DME genes experienced a whole genome duplication event (WGD) that occurred in the ancestor genome of pear and apple before their divergence based on the Ks values. Expression results showed that high salinity stress could influence the expression level of DMEs and salt-responsive genes in Pyrus betulaefolia. Furthermore, the methylation levels of salt-responsive genes changed under salt stress treatment. Results suggested important roles of PbDME genes in response to salt stress and are useful for better understanding the complex functions of this DME genes, which will facilitate epigenetic studies in pear trees salt tolerance.
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影响因子：
4.6
作者：
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影响因子：
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影响因子：
3.1
作者：
Huang, Wei;Xian, Zhiqiang;Li, Zhengguo
通讯作者：
Li, Zhengguo
DOI：
10.1073/pnas.1209329109
发表时间：
2012-08-07
影响因子：
11.1
作者：
Dowen, Robert H.;Pelizzola, Mattia;Ecker, Joseph R.
通讯作者：
Ecker, Joseph R.