In vivo microscopy of targeted vessel occlusion employing acoustic droplet vaporization.
In vivo microscopy of targeted vessel occlusion employing acoustic droplet vaporization.
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DOI：
10.1111/j.1549-8719.2012.00176.x
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Fowlkes JB
中科院分区：
文献类型：
--
作者：
Samuel S;Duprey A;Fabiilli ML;Bull JL;Fowlkes JB
栓塞术是治疗多种癌症的一种潜在手段。我们的方法-气体栓塞疗法-引入来自肿瘤上游的液滴，然后通过声学激活它们形成气泡进行闭塞-这一过程被称为声学液滴汽化(ADV)。我们想要提供第一个ADV、滞留的气泡或体内血管闭塞的光学文献。我们用大鼠提睾肌进行活体显微镜检查。将全氟碳液滴注入主动脉弓内。超声照射在生殖发育器中诱导汽化。在暴露前和暴露后对Cremaster进行ADV相关效应检查。两组实验比较了暴露在毛细血管和一级微动脉中的效果。毛细血管暴露后滞留的气泡(76μm平均长度，36μm平均直径)明显大于滋养血管暴露后的气泡(25μm平均长度，11μm平均直径)。尽管气泡大小不同，但所有滞留气泡的长度与水力直径之比为2.11(±0.65；N=112)，这与理论预测和实验观测相一致。我们的结果提供了通过ADV靶向血管闭塞的第一个光学证据。这些发现为气体栓塞术的发展奠定了基础。
Embolotherapy is a potential means to treat a variety of cancers. Our approach – gas embolotherapy – introduces the droplets upstream from the tumor and then acoustically activates them to form bubbles for occlusion – a process known as acoustic droplet vaporization (ADV). We wanted to provide the first optical documentation of ADV, lodged bubbles, or vessel occlusion in vivo. We used the rat cremaster muscle for in vivo microscopy. Perfluorocarbon droplets were administered into the aortic arch. Ultrasound exposures in the cremaster induced vaporization. The cremaster was examined pre- and post-exposure for ADV-related effects. Two sets of experiments compared the effect of exposure in the capillaries versus the first order arteriole. Bubbles that lodge following capillary exposure are significantly larger (76μm mean length, 36μm mean diameter) than those following feeder vessel exposure (25μm mean length, 11μm mean diameter). Despite the differing sizes in bubbles, the ratio of bubble length to the hydraulic diameter of all lodged bubbles was 2.11 (±0.65; N=112), which agrees with theoretical predictions and experimental observations. Our results provide the first optical evidence of targeted vessel occlusion through ADV. These findings could lay the groundwork for the advancement of gas embolotherapy.
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影响因子：
6.7
作者：
LACCOURREYE, O;LAURENT, A;MERLAND, JJ
通讯作者：
MERLAND, JJ
影响因子：
3.1
作者：
SMAJE L;ZWEIFACH B W;INTAGLIETTA M
通讯作者：
INTAGLIETTA M
影响因子：
5.5
作者：
Bakker, ENTP;Versluis, JP;VanBavel, E
通讯作者：
VanBavel, E
DOI：
10.1109/tuffc.2009.1132
发表时间：
2009-05
期刊：
IEEE transactions on ultrasonics, ferroelectrics, and frequency control
影响因子：
--
作者：
Fabiilli ML;Haworth KJ;Fakhri NH;Kripfgans OD;Carson PL;Fowlkes JB
通讯作者：
Fowlkes JB
DOI：
10.1152/ajpheart.2000.279.5.h2295
发表时间：
2000-11-01
影响因子：
4.8
作者：
Lombard, JH;Frisbee, JC;Tonellato, PJ
通讯作者：
Tonellato, PJ