Biodegradation of Poly(ethylene terephthalate) by Bacillus safensis YX8.
Biodegradation of Poly(ethylene terephthalate) by Bacillus safensis YX8.
复制标题
萨氏芽孢杆菌YX8降解聚对苯二甲酸乙酯的研究。
DOI：
10.3390/ijms242216434
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发表时间：
2023-11-17
影响因子：
5.6
通讯作者：
Yan, Xin
中科院分区：
文献类型：
--
作者：
Zeng, Caiting;Ding, Fanghui;Zhou, Jie;Dong, Weiliang;Cui, Zhongli;Yan, Xin
由于聚对苯二甲酸乙二醇酯(PET)的广泛应用，大量的PET废物排放到环境中，危害人类健康和生态。作为一种生态友好的PET废物处理方法，生物降解依赖于高效的菌株和酶。本研究首次建立了以聚己内酯(PCL)和PET纳米颗粒为底物的筛选方法。从聚酯废弃物表面分离到一株降解菌YX8。根据16S rRNA和gyrA基因的系统发育分析，该菌株被鉴定为枯草芽孢杆菌。菌株YX8能够降解PET纳米颗粒，产生对苯二甲酸(TPA)、单(2-羟乙基)对苯二甲酸(MHET)和双(2-羟乙基)对苯二甲酸(BHET)。经YX8菌株培养后，可观察到聚酯膜上的腐蚀斑点。此外，YX8菌株产生的胞外酶能够在PCL平板上形成一个清晰的区域，并能降解PET纳米颗粒生成TPA、MHET和BHET。这项工作开发了一种分离PET降解菌的方法，为PET降解和功能酶的挖掘提供了新的菌种资源。
Due to the extensive utilization of poly (ethylene terephthalate) (PET), a significant amount of PET waste has been discharged into the environment, endangering both human health and the ecology. As an eco-friendly approach to PET waste treatment, biodegradation is dependent on efficient strains and enzymes. In this study, a screening method was first established using polycaprolactone (PCL) and PET nanoparticles as substrates. A PET-degrading strain YX8 was isolated from the surface of PET waste. Based on the phylogenetic analysis of 16S rRNA and gyrA genes, this strain was identified as Bacillus safensis. Strain YX8 demonstrated the capability to degrade PET nanoparticles, resulting in the production of terephthalic acid (TPA), mono (2-hydroxyethyl) terephthalic acid (MHET), and bis (2-hydroxyethyl) terephthalic acid (BHET). Erosion spots on the PET film were observed after incubation with strain YX8. Furthermore, the extracellular enzymes produced by strain YX8 exhibited the ability to form a clear zone on the PCL plate and to hydrolyze PET nanoparticles to generate TPA, MHET, and BHET. This work developed a method for the isolation of PET-degrading microorganisms and provides new strain resources for PET degradation and for the mining of functional enzymes.
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DOI：
10.1016/j.ibiod.2018.03.017
发表时间：
2018-05-01
影响因子：
4.8
作者：
Janczak, Katarzyna;Hrynkiewicz, Katarzyna;Dabrowska, Grazyna
通讯作者：
Dabrowska, Grazyna
影响因子：
--
作者：
de Foggi, Camila C.;Machado, Ana L.;Vergani, Carlos E.
通讯作者：
Vergani, Carlos E.
影响因子：
5.6
作者：
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DOI：
10.1007/s13361-011-0244-3
发表时间：
2011-12
影响因子：
3.2
作者：
Blake SL;Walker SH;Muddiman DC;Hinks D;Beck KR
通讯作者：
Beck KR
影响因子：
5.5
作者：
Acero, Enrique Herrero;Ribitsch, Doris;Guebitz, Georg
通讯作者：
Guebitz, Georg