Translation of an engineered nanofibrous disc-like angle-ply structure for intervertebral disc replacement in a small animal model.
Translation of an engineered nanofibrous disc-like angle-ply structure for intervertebral disc replacement in a small animal model.
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DOI：
10.1016/j.actbio.2014.02.024
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发表时间：
2014-06
影响因子：
9.7
通讯作者：
Mauck, Robert L.
中科院分区：
文献类型：
--
作者：
Martin, John T.;Milby, Andrew H.;Chiaro, Joseph A.;Kim, Dong Hwa;Hebela, Nader M.;Smith, Lachlan J.;Elliott, Dawn M.;Mauck, Robert L.
腰椎间盘退变与腰痛的病因有关;然而，目前治疗症状性椎间盘疾病的手术策略有限。已经开发了多种材料来替代椎间盘组件，包括髓核（NP）、纤维环（AF）以及它们组合成椎间盘样工程构造。我们先前已经表明，电纺聚（ε-己内酯）支架层，模仿天然AF的层次组织，具有与天然组织的功能等同性。同样，我们将这些结构与细胞接种的水凝胶（作为NP替代物）组合以形成盘状角层结构（DAPS）。本研究的目的是开发一种模型，用于评价DAPS在体内。通过一系列的研究，我们开发了一种手术方法，以脱细胞DAPS替代大鼠尾侧椎间盘，然后通过外固定来稳定运动节段。然后，我们优化细胞渗透到DAPS包括牺牲聚（环氧乙烷）层散布在整个角层结构。我们的研究结果表明，DAPS是稳定的尾椎，从植入物周围空间的细胞浸润，并通过提供额外的细胞迁移途径加快浸润。这些发现建立了一个新的体内平台，在其中评估和优化功能性椎间盘置换物的设计。
Intervertebral disc degeneration has been implicated in the etiology of low back pain; however the current surgical strategies for treating symptomatic disc disease are limited. A variety of materials have been developed to replace disc components, including the nucleus pulposus (NP), the annulus fibrosus (AF), and their combination into disc-like engineered constructs. We have previously shown that layers of electrospun poly(ε-caprolactone) scaffold, mimicking the hierarchical organization of the native AF, have functional parity with native tissue. Likewise, we have combined these structures with cell-seeded hydrogels (as an NP replacement) to form disc-like angle ply structures (DAPS). The objective of this study was to develop a model for the evaluation of DAPS in vivo. Through a series of studies, we developed a surgical approach to replace the rat caudal disc with an acellular DAPS and then stabilize the motion segment by external fixation. We then optimized cell infiltration into DAPS by including sacrificial poly(ethylene oxide) layers interspersed throughout the angle-ply structure. Our findings illustrate that DAPS are stable in the caudal spine, are infiltrated by cells from the peri-implant space, and that infiltration is expedited by providing additional routes for cell migration. These findings establish a new in vivo platform in which to evaluate and optimize the design of functional disc replacements.
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影响因子：
4.1
作者：
Baker, Brendon M.;Shah, Roshan P.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.
DOI：
10.1002/jor.22355
发表时间：
2013-08
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Martin JT;Gorth DJ;Beattie EE;Harfe BD;Smith LJ;Elliott DM
通讯作者：
Elliott DM
DOI：
10.1115/1.429656
发表时间：
2000-06-01
影响因子：
1.7
作者：
Mauck, RL;Soltz, MA;Ateshian, GA
通讯作者：
Ateshian, GA
影响因子：
2.9
作者：
Bowles, Robby D.;Gebhard, Harry H.;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.
影响因子：
2.8
作者：
Korecki, Casey L.;Kuo, Catherine K.;Tuan, Rocky S.;Iatridis, James C.
通讯作者：
Iatridis, James C.