Needle puncture injury causes acute and long-term mechanical deficiency in a mouse model of intervertebral disc degeneration.
Needle puncture injury causes acute and long-term mechanical deficiency in a mouse model of intervertebral disc degeneration.
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DOI：
10.1002/jor.22355
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Elliott DM
中科院分区：
文献类型：
--
作者：
Martin JT;Gorth DJ;Beattie EE;Harfe BD;Smith LJ;Elliott DM
下背痛是一个重要的社会经济负担，椎间盘退变也是一个原因。一个可靠的椎间盘退变的动物模型是必要的，以评估治疗，和功能指标是必不可少的量化其效益。为此，在小鼠的尾椎间盘中产生针刺损伤以诱导椎间盘退变。立即和8周后评估压缩、扭转和蠕变力学，以区分损伤的影响和随后的修复或退行性反应。使用两种针头尺寸（29号和26号）来确定损伤尺寸依赖性。压缩刚度（62%），扭转刚度（60%），和早期阻尼刚度（84%）后，立即与大针（26 G）受伤。尽管结构和组成发生变化，但这些机械性能不随时间变化。在大针损伤后8周，椎间盘高度降低（37%），髓核（NP）糖胺聚糖含量降低（41%），NP胶原含量增加（45%）。小针尺寸对力学没有显著影响，并且不会引起结构和组成的退行性变化。因此，以最小的针尺寸将治疗剂注射到NP中可以限制由于针插入而造成的损伤。这些发现，沿着小鼠特异性生物探针的广泛商业可用性，表明小鼠尾椎间盘模型可以成为研究椎间盘退变和治疗的有力工具。
Low back pain is a significant socioeconomic burden and intervertebral disc degeneration has been implicated as a cause. A reliable animal model of disc degeneration is necessary to evaluate therapeutics, and functional metrics are essential to quantify their benefit. To this end, needle puncture injuries were created in the caudal intervertebral discs of mice to induce disc degeneration. Compression, torsion, and creep mechanics were assessed both immediately and after eight weeks to distinguish between the effects of injury and the subsequent reparative or degenerative response. Two needle sizes (29 and 26 gauge) were used to determine injury size-dependence. Compressive stiffness (62%), torsional stiffness (60%), and early damping stiffness (84%) decreased immediately after injury with the large needle (26G). These mechanical properties did not change over time despite structural and compositional changes. At 8 weeks following large needle injury, disc height decreased (37%), nucleus pulposus (NP) glycosaminoglycan content decreased (41%), and NP collagen content increased (45%). The small needle size had no significant effect on mechanics and did not initiate degenerative changes in structure and composition. Thus, the injection of therapeutics into the NP with a minimal needle size may limit damage due to the needle insertion. These findings, along with the wide commercial availability of mouse-specific biological probes, indicate that the mouse caudal disc model can be a powerful tool for investigating disc degeneration and therapy.
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DOI：
10.1016/j.spinee.2006.04.014
发表时间：
2006-11-01
期刊：
The spine journal : official journal of the North American Spine Society
影响因子：
--
作者：
Miyamoto, Kei;Masuda, Koichi;An, Howard S
通讯作者：
An, Howard S
影响因子：
2.9
作者：
FARNDALE, RW;SAYERS, CA;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
3
作者：
Masuda, K;Aota, Y;An, HS
通讯作者：
An, HS
局部椎间盘损伤会导致结构，细胞和生物合成的器官水平变化。
影响因子：
2.8
作者：
Iatridis, James C.;Michalek, A. J.;Purmessur, D.;Korecki, C. L.
通讯作者：
Korecki, C. L.
影响因子：
4.9
作者：
Urban JP;Roberts S
通讯作者：
Roberts S