Phospholipase D-mediated hypersensitivity at central synapses is associated with abnormal behaviours and pain sensitivity in rats exposed to prenatal stress.
Phospholipase D-mediated hypersensitivity at central synapses is associated with abnormal behaviours and pain sensitivity in rats exposed to prenatal stress.
复制标题
磷脂酶 D 介导的中央突触超敏反应与暴露于产前应激的大鼠的异常行为和疼痛敏感性有关。
DOI：
10.1016/j.biocel.2013.07.017
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sun L
中科院分区：
文献类型：
--
作者：
Sun L
发育关键阶段的不良事件可能导致中枢神经系统（CNS）持久功能障碍。为了寻找突触功能潜在的潜在变化，我们使用了一种新开发的方案来测量突触神经体受体介导的 Ca2+ 荧光反应的变化，这些突触神经体是从与情绪和疼痛处理有关的 CNS 选定区域中新鲜分离出来的。我们比较了成年雄性对照和妊娠晚期暴露于社会压力（产前应激，PS）的大鼠后代，结果显示出程序性行为变化，表明焦虑、快感缺失和疼痛过敏。我们发现，与正常对照组相比，PS 大鼠的突触神经体从额叶皮层对谷氨酸受体 (GluR) 激动剂以及从脊髓对伤害性传入激活剂的反应性相应增加。通过药理学和生化相结合的策略，我们发现了磷脂酶 D1 (PLD1) 介导的信号传导作用的证据，该信号传导可能涉及神经系统两个水平的 5-HT2A 受体 (5-HT2AR) 激活。这些变化可能参与支撑 PS 引起的持久行为改变。
Adverse events at critical stages of development can lead to lasting dysfunction in the central nervous system (CNS). To seek potential underlying changes in synaptic function, we used a newly developed protocol to measure alterations in receptor-mediated Ca2+fluorescence responses of synaptoneurosomes, freshly isolated from selected regions of the CNS concerned with emotionality and pain processing. We compared adult male controls and offspring of rats exposed to social stress in late pregnancy (prenatal stress, PS), which showed programmed behavioural changes indicating anxiety, anhedonia and pain hypersensitivity. We found corresponding increases, in PS rats compared with normal controls, in responsiveness of synaptoneurosomes from frontal cortex to a glutamate receptor (GluR) agonist, and from spinal cord to activators of nociceptive afferents. Through a combined pharmacological and biochemical strategy, we found evidence for a role of phospholipase D1 (PLD1)-mediated signalling, that may involve 5-HT2Areceptor (5-HT2AR) activation, at both levels of the nervous system. These changes might participate in underpinning the enduring alterations in behaviour induced by PS.
登录
查看更多内容
影响因子：
14.8
作者：
Scott, Sarah A.;Selvy, Paige E.;Buck, Jason R.;Cho, Hyekyung P.;Criswell, Tracy L.;Thomas, Ashley L.;Armstrong, Michelle D.;Arteaga, Carlos L.;Lindsley, Craig W.;Brown, H. Alex
通讯作者：
Brown, H. Alex
在成年雌性和雄性大鼠的福尔马林测试中，母体压力不同地改变伤害感受行为
影响因子：
2.9
作者：
I. Butkevich;E. Vershinina
通讯作者：
E. Vershinina
产前应激和新生儿处理之间的相互作用对雄性和雌性大鼠伤害性反应潜伏期的影响
影响因子：
2.9
作者：
J. Smythe;C. McCormick;J. Rochford;M. Meaney
通讯作者：
M. Meaney
影响因子：
7.3
作者：
D. Pellegrini-Giampietro;Serenella Albani Torregrossa;F. Moroni
通讯作者：
D. Pellegrini-Giampietro;Serenella Albani Torregrossa;F. Moroni
产前应激对成年雄性大鼠福尔马林引起的急性和持续性疼痛的影响
影响因子：
0.7
作者：
I. Butkevich
通讯作者：
I. Butkevich