Injectable hydrogel incorporating with nanoyarn for bone regeneration
Injectable hydrogel incorporating with nanoyarn for bone regeneration
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与纳米纱线结合的可注射水凝胶用于骨再生
DOI：
10.1080/09205063.2013.848326
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Mo X.
中科院分区：
文献类型：
--
作者：
Wu T.;Ramakrishna S.;Liao S.;Mo X.
传统的骨移植需要开放的手术方法来移植应用部位，随之而来的并发症是手术疤痕大，疼痛增加，术后恢复时间较长。为了克服这些局限性，迫切需要开发更好的骨移植替代物。在这项研究中，我们开发了一种新型的可注射系统，该系统是由聚（l -乳酸-co-ε-己内酯）（P(la - cl)）纳米线悬浮在I型胶原水凝胶（Col）中组成的仿生骨替代品。采用动态液体支撑系统制备连续P（LLA-CL）纳米纱。将电纺丝的长纳米纱在掺入Col之前剪短成短纳米纱，流变学评价结果表明，掺入纳米纱后，Col的力学性能得到了提高。低温与纳米纱的混合物可以从16号针中顺利注入。体外研究表明，人间充质干细胞（hMSCs）在纳米流细胞上增殖良好。纳米颗粒水凝胶处理后，hMSCs的碱性磷酸酶活性和骨钙素表达明显高于对照组。这项研究强调了使用一种新型可注射仿生支架进行骨再生的潜力。
Traditional bone grafting requires an open surgical approach to the graft application sites with the attendant complications of a large surgical scar, increased pain and a longer post-operative recovery. To overcome these limitations, there is a great need for the development of better bone graft substitutes. In this study, we developed a novel injectable system which was a biomimetic bone substitute consisted of Poly (L-lactide-co-ε-caprolactone) (P(LLA-CL)) nanoyarns suspended in type I collagen hydrogel (Col). A dynamic liquid support system was employed to fabricate continuous P(LLA-CL) nanoyarns. The electrospun long nanoyarns were chopped into short nanoyarns before they were incorporated into Col. The result of rheological evaluation showed that the mechanical property of Col was enhanced after the nanoyarns were incorporated into it. The mixture of Col and nanoyarn could be smoothly injected out of 16 gauge needle. In vitro study showed that human mesenchymal stem cells (hMSCs) proliferated well on Col with nanoyarns. Alkaline phosphatase activity and osteocalcin expression of hMSCs on hydrogel with nanoyarns were much higher than those on control groups. This study highlights the potential of using a novel injectable biomimetic scaffold for bone regeneration.
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影响因子：
3.2
作者：
SungCheal Moon;R. Farris
通讯作者：
SungCheal Moon;R. Farris
影响因子：
--
作者：
Jun Xie;Maki Ihara;Youngmee Jung;I. Kwon;S. Kim;Young Ha Kim;T. Matsuda
通讯作者：
Jun Xie;Maki Ihara;Youngmee Jung;I. Kwon;S. Kim;Young Ha Kim;T. Matsuda
影响因子：
3
作者：
Anna Thorvaldsson;J. Silva-Correia;J. Oliveira;R. Reis;P. Gatenholm;P. Walkenström
通讯作者：
Anna Thorvaldsson;J. Silva-Correia;J. Oliveira;R. Reis;P. Gatenholm;P. Walkenström
影响因子：
3.4
作者：
Dikovsky, Daniel;Bianco-Peled, Havazelet;Seliktar, Dror
通讯作者：
Seliktar, Dror
影响因子：
14
作者：
Mo, XM;Xu, CY;Ramakrishna, S
通讯作者：
Ramakrishna, S