Structural characterization and hepatoprotective activity of an acidic polysaccharide from Ganoderma lucidum.
Structural characterization and hepatoprotective activity of an acidic polysaccharide from Ganoderma lucidum.
复制标题
灵芝酸性多糖的结构表征及其保肝活性
DOI：
10.1016/j.fochx.2022.100204
复制
发表时间：
2022-03-30
期刊：
影响因子：
6.1
通讯作者：
Wu, Qingping
中科院分区：
文献类型：
--
作者：
Chen, Shaodan;Guan, Xiaoying;Yong, Tianqiao;Gao, Xiong;Xiao, Chun;Xie, Yizhen;Chen, Diling;Hu, Huiping;Wu, Qingping
关键词：
灵芝粗多糖（GLP）对束缚应激所致小鼠肝损伤具有保护作用，其机制可能与改善氧化状态有关。通过阴离子交换层析（AEC）和凝胶过滤从GLP中纯化两种多糖，包括中性β-葡聚糖和含有葡萄糖醛酸的酸性β-葡聚糖。酸性多糖的体外保肝作用强于中性多糖。阴离子交换色谱（AEC）是利用不同离子强度缓冲液将β-葡聚糖分离成中性和离子组分的有效方法。灵芝粗多糖（GLP）可通过改善氧化状态对束缚应激所致小鼠肝损伤具有保护作用。采用阴离子交换色谱（AEC）结合凝胶渗透法从灵芝多糖中分离纯化了中性β-葡聚糖（GLPB 2）和酸性β-葡聚糖（GLPC 2）。GLPC 2的平均分子量为20.56 kDa，与GLPB 2相比，GLPC 2对H2 O2诱导的HepG 2细胞肝损伤具有更强的肝保护作用。糖苷残基和NMR分析表明GLPC 2含有d-Glcp-（1→，→3）-d-Glcp-（1→，→4）-d-Glcp-（1→，→6）-d-Glcp-（1→，→3，6）-d-Glcp-（1 →和→ 4）-d-GlcpA-（1 →）。利用不同离子强度的缓冲液，AEC可有效地将β-葡聚糖分离成中性和酸性组分。研究结果为G.灵芝多糖在食品和医药工业中具有保肝作用。
Ganoderma lucidum crude polysaccharide (GLP) exhibited protective effect on liver damage in mice caused by restraint stress through improving oxidative status. Two polysaccharides, including a neutral β-glucan and an acidic β-glucan containing glucuronic acid were purified from GLP by anion-exchange chromatography (AEC) and gel filtration. Acidic polysaccharide demonstrated stronger hepatoprotective effect in vitro compared to neutral polysaccharide. Anion-exchange chromatography (AEC) is an effective technique for separate β-glucan into neutral and ionic fractions by different ionic strength buffer. In this study, Ganoderma lucidum crude polysaccharide (GLP) was found to have protective effect on liver damage in mice caused by restraint stress through improving oxidative status. Two polysaccharides, including a neutral β-glucan (GLPB2) and an acidic β-glucan (GLPC2) were purified from GLP through anion-exchange chromatography (AEC) combined with gel permeation. GLPC2, with an average molecular weight of 20.56 kDa, exhibited stronger hepatoprotective effect against H2O2-induced liver injury in HepG2 cells compared to GLPB2. Glycosidic residues and NMR analysis comprehensively revealed that GLPC2 contained d-Glcp-(1→, →3)-d-Glcp-(1→, →4)-d-Glcp-(1→, →6)-d-Glcp-(1→, →3, 6)-d-Glcp-(1 → and → 4)-d-GlcpA-(1 → . AEC can be an effective technique for separating β-glucans into neutral and acidic fractions by different ionic strength buffer. The findings provided a theoretical basis for the potential application of G. lucidum polysaccharides as a hepatoprotective in food and pharmaceutical industry.
登录
查看更多内容
影响因子：
5.5
作者：
Chen S;Yong T;Zhang Y;Su J;Jiao C;Xie Y
通讯作者：
Xie Y
DOI：
10.1016/j.ijbiomac.2010.10.015
发表时间：
2011-01-01
影响因子：
8.2
作者：
Huang, Sheng-quan;Li, Jin-wei;Wang, Zhou
通讯作者：
Wang, Zhou
影响因子：
7.4
作者：
KIM, JB;CARPITA, NC
通讯作者：
CARPITA, NC
DOI：
10.1016/j.ijbiomac.2019.09.141
发表时间：
2020-01-15
影响因子：
8.2
作者：
Li, Jia;Gu, Feifei;Yu, Guangli
通讯作者：
Yu, Guangli
影响因子：
3.1
作者：
Dong, Qun;Wang, Ying;Ding, Kan
通讯作者：
Ding, Kan