Molecular characterization of avian leukosis virus subgroup J in Chinese local chickens between 2013 and 2018.
Molecular characterization of avian leukosis virus subgroup J in Chinese local chickens between 2013 and 2018.
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2013-2018年中国本土鸡禽白血病病毒J亚群的分子特征
DOI：
10.1016/j.psj.2020.08.004
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发表时间：
2020-11
期刊：
影响因子：
4.4
通讯作者：
Gao Y
中科院分区：
文献类型：
--
作者：
Ma M;Yu M;Chang F;Xing L;Bao Y;Wang S;Farooque M;Li X;Liu P;Chen Y;Qi X;Pan Q;Gao L;Li K;Liu C;Zhang Y;Cui H;Wang X;Sun Y;Gao Y
禽白血病病毒J亚型（ALV-J）于1999年首次从我国肉鸡中分离得到；随后，它被迅速引入蛋鸡和中国土鸡中。近年来，肉鸡和蛋鸡中ALV-J的发病率明显下降。然而，它却给中国本土鸡造成了巨大的损害，给当地鸡的生产性能和养殖安全带来了巨大的挑战。为了系统分析中国地方鸡ALV-J的分子特征和流行趋势，收集了2013-2018年260份临床样本；通过抗原捕获酶联免疫吸附测定和亚组 A、B 和 J 特异性多重 PCR 鉴定了 18 个 ALV-J 本地鸡分离株。通过PCR扩增18个分离株的全基因组序列并提交给GenBank。大约 18 个分离株中的 55.5% (10/18) 与 20 个 ALV-J 早期分离的本地毒株（从 GenBank 获得的基因组序列）在 gp85 基因聚集在一个单独的分支中表现出相对较高的同源性 (92.3–95.4%)。 18个分离株的3′非翻译区（3′UTR）在冗余跨膜区和直接重复区1中分别显示出195-210和16-28个碱基对缺失； 18 个分离株中的 55.5% (10/18) 保留了 147 个残基 E 元件。 18株分离株中61.1%（11/18）的U3基因与ALV-J早期分离的地方株具有较高的一致性（94.6%~97.3%）。这些结果表明ALV-J地方鸡分离株的gp85和U3已经迅速进化并形成了独特的地方鸡分支。此外，目前已确定3'UTR区基因缺失是我国ALV-J独有的分子特征。因此，所获得的结果基于现有的ALV-J分子流行病学数据，进一步阐明了ALV-J在中国地方鸡中的遗传进化趋势。
Avian leukosis virus subgroup J (ALV-J) was first isolated from broiler chickens in China in 1999; subsequently, it was rapidly introduced into layer chickens and Chinese local chickens. Recently, the incidence of ALV-J in broiler and layer chickens has significantly decreased. However, it has caused substantial damage to Chinese local chickens, resulting in immense challenges to their production performance and breeding safety. To systematically analyze the molecular characteristics and the epidemic trend of ALV-J in Chinese local chickens, 260 clinical samples were collected for the period of 2013–2018; 18 ALV-J local chicken isolates were identified by antigen-capture enzyme-linked immunosorbent assay and subgroup A-, B-, and J-specific multiplex PCR. The whole genomic sequences of 18 isolates were amplified with PCR and submitted to GenBank. Approximately, 55.5% (10/18) of the 18 isolates demonstrated a relatively high homology (92.3–95.4%) with 20 ALV-J early-isolated local strains (genome sequences obtained from GenBank) in gp85 genes clustering in a separated branch. The 3ʹ untranslated region (3ʹ UTR) of the 18 isolates showed a 195–210 and 16–28 base pair deletion in the redundant transmembrane region and in direct repeat 1, respectively; 55.5% (10/18) of the 18 isolates retained the 147 residue E element. The U3 gene of 61.1% (11/18) of the 18 isolates shared high identity (94.6–97.3%) with ALV-J early-isolated local strains. These results implied that the gp85 and U3 of ALV-J local chicken isolates have rapidly evolved and formed a unique local chicken branch. In addition, it was determined that the gene deletion in the 3′UTR region currently serves as a unique molecular characteristic of ALV-J in China. Hence, the obtained results built on the existing ALV-J molecular epidemiological data and further elucidated the genetic evolution trend of ALV-J in Chinese local chickens.
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影响因子：
5.3
作者：
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通讯作者：
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影响因子：
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影响因子：
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影响因子：
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作者：
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