Sub-retinal drusenoid deposits in human retina: organization and composition.
Sub-retinal drusenoid deposits in human retina: organization and composition.
复制标题
DOI：
10.1016/j.exer.2008.07.010
复制
发表时间：
2008-11
影响因子：
3.4
通讯作者：
Curcio, Christine A.
中科院分区：
文献类型：
--
作者：
Rudolf, Martin;Malek, Goldis;Messinger, Jeffrey D.;Clark, Mark E.;Wang, Lan;Curcio, Christine A.
关键词：
我们在三只老年人眼中展示了组织学上的视网膜下玻璃疣碎片，其中两只眼睛患有与年龄相关的黄斑病。通过死后眼底检查，病变呈玻璃膜疣样，即明显位于视网膜色素上皮（RPE）水平的苍白斑点。光学和电子显微镜显示视网膜下腔中膜碎片的聚集，这是软玻璃疣的主要成分。免疫组织化学和共聚焦显微镜证实了通常与玻璃膜疣（未酯化胆固醇、apoE、补体因子 H 和玻连蛋白呈阳性）相关的分子的存在，但没有证据表明与光感受器（凝集素和视蛋白）、Müller 细胞（胶质纤维酸性蛋白和细胞视网膜结合蛋白，CRALPB）或 RPE (CRALPB) 相关的分子。事实上，类玻璃膜疣物质与传统玻璃膜疣共享一些标记，可以出现在 RPE 的相对面上，这表明 RPE 基底外侧分泌的正常高度矢量过程的极性紊乱，并且分泌物质的过量产生和分泌方向是独立指定的过程。未来，新兴的高分辨率成像技术可能会更频繁地揭示玻璃疣视网膜下碎片。
We demonstrate histologically sub-retinal drusenoid debris in three aged human eyes, two of them affected by age-related maculopathy. By postmortem fundus examination, the lesions appeared drusen-like, i.e., they were pale spots apparently at the level of the retinal pigment epithelium (RPE). Light and electron microscopy revealed aggregations of membranous debris, the principal constituent of soft drusen, in the sub-retinal space. Immunohistochemistry and confocal microscopy confirmed the presence of molecules typically associated with drusen (positive for unesterified cholesterol, apoE, complement factor H, and vitronectin) without evidence for molecules associated with photoreceptors (lectins and opsins), Müller cells (glial fibrillary acid protein and cellular retinal binding protein, CRALPB), or RPE (CRALPB). The fact that a drusenoid material, sharing some markers with conventional drusen, can occur on opposite faces of the RPE, suggests deranged polarity of normally highly vectorial processes for basolateral secretion from RPE, and that overproduction of secreted materials and direction of secretion are independently specified processes. In the future, drusenoid sub-retinal debris might be more frequently revealed by emerging high-resolution imaging techniques.
登录
查看更多内容
影响因子：
3.4
作者：
Bonilha, VL;Bhattacharya, SK;Crabb, JW
通讯作者：
Crabb, JW
DOI：
10.1073/pnas.0501536102
发表时间：
2005-05-17
影响因子：
11.1
作者：
Hageman, GS;Anderson, DH;Allikmets, R
通讯作者：
Allikmets, R
DOI：
10.1073/pnas.222551899
发表时间：
2002-11-12
影响因子：
11.1
作者：
Crabb, JW;Miyagi, M;Hollyfield, JG
通讯作者：
Hollyfield, JG
影响因子：
3.2
作者：
GUYTON, JR;KLEMP, KF
通讯作者：
KLEMP, KF
影响因子：
4.1
作者：
Pieroni, CG;Witkin, AJ;Duker, JS
通讯作者：
Duker, JS