Dynamic interplay among monocyte-derived, dermal, and resident lymph node dendritic cells during the generation of vaccine immunity to fungi.
Dynamic interplay among monocyte-derived, dermal, and resident lymph node dendritic cells during the generation of vaccine immunity to fungi.
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DOI：
10.1016/j.chom.2010.05.010
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发表时间：
2010-06-25
影响因子：
30.3
通讯作者：
Klein BS
中科院分区：
文献类型：
--
作者：
Ersland K;Wüthrich M;Klein BS
能够引发抗真菌CD4 T细胞的早期先天事件知之甚少。我们设计了一种具有模型表位EαRFP的减毒真菌疫苗，以在酵母识别、Ag呈递和初始T细胞引发期间全面跟踪疫苗对皮肤炎芽生菌的免疫。皮下注射疫苗后，单核细胞衍生的炎性树突状细胞（DC）是最早和最大的群体，与酵母菌，携带它们进入引流节点。尽管与酵母显著相关，但这些DC不能展示表面肽：MHC复合物或初始幼稚T细胞。相反，展示Ag和致敏CD4 T细胞的能力存在于从迁移DC转移Ag后的LN驻留DC和皮肤迁移DC中。我们的工作揭示了不同DC亚群之间的动态相互作用，这些亚群在酵母菌注射到皮肤中后引发幼稚CD4 T细胞，并揭示了诱导疫苗对真菌免疫的细胞成分。
Early innate events that enable priming of anti-fungal CD4 T cells are poorly understood. We engineered an attenuated fungal vaccine with a model epitope EαRFP to track vaccine immunity to Blastomyces dermatitidis comprehensively during yeast recognition, Ag presentation, and priming of naïve T cells. After subcutaneous injection of the vaccine, monocyte-derived inflammatory dendritic cells (DCs) are the earliest and largest population that associates with yeast, carrying them into the draining nodes. Despite marked association with yeast, these DCs fail to display surface peptide:MHC complexes or prime naïve T cells. Instead, the ability to display Ag and prime CD4 T cells resides with LN resident DCs after Ag transfer from immigrant DCs, and with skin migratory DCs. Our work reveals the dynamic interplay among distinct DC subsets that prime naïve CD4 T cells after yeast are injected in the skin, and discloses the cellular elements that induce vaccine immunity to fungi.
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影响因子：
30.3
作者：
Hohl TM;Rivera A;Lipuma L;Gallegos A;Shi C;Mack M;Pamer EG
通讯作者：
Pamer EG
影响因子：
4.6
作者：
Probst, HC;Tschannen, K;Van Den Broek, M
通讯作者：
Van Den Broek, M
影响因子：
64.8
作者：
RUDENSKY, AY;RATH, S;JANEWAY, CA
通讯作者：
JANEWAY, CA
DOI：
10.1084/jem.20080601
发表时间：
2009-02-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lee HK;Zamora M;Linehan MM;Iijima N;Gonzalez D;Haberman A;Iwasaki A
通讯作者：
Iwasaki A
DOI：
10.4049/jimmunol.0902823
发表时间：
2009-12-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Osterholzer JJ;Chen GH;Olszewski MA;Curtis JL;Huffnagle GB;Toews GB
通讯作者：
Toews GB