Aptamer-based fluorometric determination for mucin 1 using gold nanoparticles and carbon dots
Aptamer-based fluorometric determination for mucin 1 using gold nanoparticles and carbon dots
复制标题
使用金纳米粒子和碳点进行基于适体的粘蛋白 1 荧光测定
DOI：
10.1007/s00604-019-3516-4
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发表时间：
2019-07
影响因子：
5.7
通讯作者：
Yan Chao
中科院分区：
文献类型：
--
作者：
Wang Weiwei;Wang Yan;Pan Hong;Cheddah Soumia;Yan Chao
描述了一种测定粘蛋白1 （MUC1）的荧光法。它是基于抗MUC1适配体对MUC1的特异性结合，以及利用金纳米颗粒（AuNPs）对碳点（cd）蓝色荧光的内部过滤效应（IFE）。当CDs与AuNPs混合时，它们的荧光由于IFE而降低。fe的效率可以通过AuNPs的吸附和聚集状态来调节。后者可以通过添加盐来诱导，从而使CDs的荧光恢复。适体吸附在AuNPs上，保护AuNPs免受盐诱导的聚集，并伴有从红色到蓝色的颜色变化。如果在盐的存在下将适体加入到CDs和AuNPs的混合物中，则会抑制AuNPs的聚集。因此，CDs的蓝色荧光（在激发/发射波长为365/448 nm时测量效果最好）被降低。然而，如果适配体结合MUC1，适配体将从aunp表面释放。这降低了aunp的耐盐性，并导致蓝色荧光的恢复。荧光强度随MUC1浓度的增加而增加。该方法在5.3 ~ 200 ng·mL−1 muc1浓度范围内具有良好的线性响应，检出限为5.3 ng·mL−1。该方法对MUC1对其他蛋白具有良好的选择性。(1)游离AuNPs和NaCl聚集的AuNPs的IFE影响示意图；(2)MUC1与适配体的特异性相互作用对NaCl诱导的AuNPs聚集和IFE效率的影响示意图。
A fluorometric method is described for the determination of mucin 1 (MUC1). It is based on the specific binding of MUC1 by anti-MUC1 aptamers and by exploiting the inner filter effect (IFE) exerted by gold nanoparticles (AuNPs) on the blue fluorescence of carbon dots (CDs). When CDs are mixed with AuNPs, their fluorescence is reduced due to an IFE. The IFE efficiency can be modulated by the adsorption and the aggregation state of AuNPs. The latter can be induced by addition of salt, thereby allowing the fluorescence of the CDs to recover. The aptamer is adsorbed on the AuNPs and protects the AuNPs from salt-induced aggregation which is accompanied by a color change from red to blue. If aptamer is added to a mixture of CDs and AuNPs in presence of salt, the aggregation of the AuNPs is inhibited. Thus, the blue fluorescence of the CDs (best measured at excitation/emission wavelengths of 365/448 nm) is reduced. If, however, the aptamers bind MUC1, the aptamers will be released from the surface of the AuNPs. This decreases the salt tolerance of AuNPs and leads to the recovery of the blue fluorescence. The fluorescence intensity increases with the concentration of MUC1. The method has a linear response in the 5.3 to 200 ng·mL−1MUC1 concentration range and a lower detection limit of 5.3 ng·mL−1. The method displays excellent selectivity towards MUC1 against other proteins.Graphical abstractSchematic illustration of (I) the influence of IFE of free AuNPs and NaCl aggregated AuNPs and (II) the influence of specific interaction between MUC1 and aptamers on the NaCl-induced aggregation of AuNPs and the IFE efficiency.
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DOI：
--
发表时间：
1998-02
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
S. Zhang;H. S. Zhang;V. Reuter;S. Slovin;H. Scher;P. Livingston
通讯作者：
S. Zhang;H. S. Zhang;V. Reuter;S. Slovin;H. Scher;P. Livingston
影响因子：
5.7
作者：
Ma, Nan;Jiang, Wentao;Yang, Minghui
通讯作者：
Yang, Minghui
影响因子：
2.7
作者：
Seonmi Shin;Hye Yeon Nam;Eun Jeong Lee;W. Jung;S. Hah
通讯作者：
Seonmi Shin;Hye Yeon Nam;Eun Jeong Lee;W. Jung;S. Hah
影响因子：
7.4
作者：
Cheng, Alan K. H.;Su, Huaipeng;Yu, Hua-Zhong
通讯作者：
Yu, Hua-Zhong
影响因子：
11.2
作者：
M. Nacht;A. Ferguson;Wen Zhang;J. Petroziello;B. Cook;Yu Hong Gao;Sharon Maguire;Deborah Riley;George Coppola;G. Landes;S. Madden;S. Sukumar
通讯作者：
M. Nacht;A. Ferguson;Wen Zhang;J. Petroziello;B. Cook;Yu Hong Gao;Sharon Maguire;Deborah Riley;George Coppola;G. Landes;S. Madden;S. Sukumar