Genome-Wide Identification of Dicer-Like, Argonaute, and RNA-Dependent RNA Polymerase Gene Families in Brassica Species and Functional Analyses of Their Arabidopsis Homologs in Resistance to Sclerotinia sclerotiorum.
Genome-Wide Identification of Dicer-Like, Argonaute, and RNA-Dependent RNA Polymerase Gene Families in Brassica Species and Functional Analyses of Their Arabidopsis Homologs in Resistance to Sclerotinia sclerotiorum.
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芸苔属物种 Dicer 样、Argonaute 和 RNA 依赖性 RNA 聚合酶基因家族的全基因组鉴定及其拟南芥同源物抗核盘菌的功能分析
DOI：
10.3389/fpls.2016.01614
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发表时间：
2016
影响因子：
5.6
通讯作者：
Cai XZ
中科院分区：
文献类型：
--
作者：
Cao JY;Xu YP;Li W;Li SS;Rahman H;Cai XZ
RNA沉默是植物基因表达调控和抗病毒防御的重要机制。然而，重要油料作物甘蓝型油菜中的RNA沉默机制以及在抗毁灭性真菌病原体核盘菌（Sclerotinia sclerotiorum）中的功能还不清楚。本研究对B. napus的抗性，并探讨了它们在抗S.显示了核盘菌。异源多倍体物种B的基因组。甘蓝型油菜具有8个Dicer-like（DCL）基因、27个Argonaute（AGO）基因和16个依赖RNA的RNA聚合酶（RDR）基因，这些基因几乎包括了其祖先种B的所有拷贝。rapa和B.结果表明，RDR 5基因在B.在进化过程中，油菜似乎通过复制而进一步扩大。与拟南芥相比，AGO 1、AGO 4、AGO 9和RDR 5等AGO和RDR基因在这些芸苔属植物中均得到了显著扩增。在51个DCL、AGO和RDR基因中，有21个含有钙调素结合转录激活因子（CAMTA）结合位点（CGCG box）。S.核盘菌接种强烈诱导BnCAMTA 3基因的表达，同时显著抑制一些含有CGCG的RNA沉默组分基因的表达，表明RNA沉默机制可能是CAMTA 3的靶向。此外，拟南芥突变体分析表明，dcl 4 -2，ago 9 -1，rdr 1 -1，rdr 6 - 1 - 1和rdr 6 -15突变体对S.而dcl 1 -9的抗性较强。我们的研究结果揭示了RNA沉默在植物抗S.揭示了CAMTA功能和RNA沉默调控的新机制。
RNA silencing is an important mechanism to regulate gene expression and antiviral defense in plants. Nevertheless, RNA silencing machinery in the important oil crop Brassica napus and function in resistance to the devastating fungal pathogen Sclerotinia sclerotiorum are not well-understood. In this study, gene families of RNA silencing machinery in B. napus were identified and their role in resistance to S. sclerotiorum was revealed. Genome of the allopolyploid species B. napus possessed 8 Dicer-like (DCL), 27 Argonaute (AGO), and 16 RNA-dependent RNA polymerase (RDR) genes, which included almost all copies from its progenitor species B. rapa and B. oleracea and three extra copies of RDR5 genes, indicating that the RDR5 group in B. napus appears to have undergone further expansion through duplication during evolution. Moreover, compared with Arabidopsis, some AGO and RDR genes such as AGO1, AGO4, AGO9, and RDR5 had significantly expanded in these Brassica species. Twenty-one out of 51 DCL, AGO, and RDR genes were predicted to contain calmodulin-binding transcription activators (CAMTA)-binding site (CGCG box). S. sclerotiorum inoculation strongly induced the expression of BnCAMTA3 genes while significantly suppressed that of some CGCG-containing RNA silencing component genes, suggesting that RNA silencing machinery might be targeted by CAMTA3. Furthermore, Arabidopsis mutant analyses demonstrated that dcl4-2, ago9-1, rdr1-1, rdr6-11, and rdr6-15 mutants were more susceptible to S. sclerotiorum, while dcl1-9 was more resistant. Our results reveal the importance of RNA silencing in plant resistance to S. sclerotiorum and imply a new mechanism of CAMTA function as well as RNA silencing regulation.
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通讯作者：
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