Simultaneous analysis of acetaminophen, p-aminophenol and aspirin metabolites by hydrophilic interaction and strong anion exchange capillary liquid chromatography coupled to amperometric detection.
Simultaneous analysis of acetaminophen, p-aminophenol and aspirin metabolites by hydrophilic interaction and strong anion exchange capillary liquid chromatography coupled to amperometric detection.
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通过亲水相互作用和强阴离子交换毛细管液相色谱与安培检测相结合，同时分析对乙酰氨基酚、对氨基苯酚和阿司匹林代谢物。
DOI：
10.1002/jssc.201100163
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发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
Xiaoping Wu*
中科院分区：
文献类型：
--
作者：
Minmin Zheng;Yimin Wu;Lanxiang Lu;Kang Ding;Fengxiang Tang;Zian Lin;Xiaoping Wu*
建立了一种同时测定对乙酰氨基酚、对氨基酚和阿司匹林代谢产物水杨酸、龙胆酸、水杨酸尿酸和2，3-二羟基苯甲酸等极性非甾体药物及其代谢产物的毛细管液相色谱安培检测方法。采用以乙腈和Tris缓冲液为移动的流动相和混合模式固定相的毛细管整体柱，在8 min内实现了6种极性分析物的快速分离，表现出亲水作用和强阴离子交换分离机理。方法检测限为10 ~ 50 ng/mL。在精密度方面，所有分析物的日内和日间相对标准偏差值均未超过3.1%（迁移时间）和8.9%（峰面积）。该方法为极性药物的分析提供了一种简便、快速、经济的方法。该方法在药代动力学中的适用性通过用化合物加标人血清样品并分析回收率来验证。
A simple and sensitive method has been developed for the simultaneous determination of polar nonsteroidal pharmaceuticals and metabolites, including acetaminophen, p-aminophenol and several aspirin metabolites (salicylic acid, gentisic acid, salicyluric acid and 2,3-dihydroxybenzoic acid), by capillary liquid chromatography with amperometric detection. Using a capillary monolithic column with mixed mode stationary phases and a mobile phase composed of acetonitrile and Tris buffer, rapid separation of six polar analytes was achieved within 8 min, and a hydrophilic interaction and strong anion exchange separation mechanism were exhibited. Method detection limits of six analytes ranged from 10 to 50 ng/mL. In terms of precision, the intra- and interday relative standard deviation values in all analytes never exceeded 3.1% for migration time and 8.9% for peak areas, respectively. This method provided a simple, rapid and cost-effective approach for the analysis of polar pharmaceuticals. The applicability of the method in pharmacokinetics was verified by spiking human serum samples with the compounds and analyzing the recoveries.
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影响因子：
--
作者：
S. Khan;Muhammad Hamayun;Arif-ullah Khan;B. Ahmad;Safia Ahmed;In-Jung Lee
通讯作者：
S. Khan;Muhammad Hamayun;Arif-ullah Khan;B. Ahmad;Safia Ahmed;In-Jung Lee
电化学固相微萃取循环伏安法和微分脉冲伏安法测定血样中的水杨酸
影响因子：
2.5
作者：
Guan, Xinyan;Ji, Hongguo;Zhu, Yongchun
通讯作者：
Zhu, Yongchun
影响因子：
2.2
作者：
Takeshi Saito;S. Morita;S. Inoue;I. Yamamoto;S. Inokuchi
通讯作者：
Takeshi Saito;S. Morita;S. Inoue;I. Yamamoto;S. Inokuchi
DOI：
10.1002/rcm.2748
发表时间：
2006-11
期刊：
Rapid communications in mass spectrometry : RCM
影响因子：
--
作者：
F. Vinci;Serena Fabbrocino;M. Fiori;L. Serpe;P. Gallo
通讯作者：
F. Vinci;Serena Fabbrocino;M. Fiori;L. Serpe;P. Gallo
影响因子：
3.1
作者：
Naoki Igawa;S. Kitagawa;H. Ohtani
通讯作者：
Naoki Igawa;S. Kitagawa;H. Ohtani