Postnatal expression of V2 vasopressin receptor splice variants in the rat cerebellum.
Postnatal expression of V2 vasopressin receptor splice variants in the rat cerebellum.
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DOI：
10.1016/j.diff.2008.11.002
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发表时间：
2009-04
期刊：
影响因子：
2.9
通讯作者：
Gonzalez, Carlos B.
中科院分区：
文献类型：
--
作者：
Vargas, Karina J.;Sarmiento, Jose M.;Ehrenfeld, Pamela;Anazco, Carolina C.;Villanueva, Carolina I.;Carmona, Pamela L.;Brenet, Marianne;Navarro, Javier;Mueller-Esterl, Werner;Gonzalez, Carlos B.
V2加压素受体基因在外显子3中含有一个选择性剪接位点，这导致产生两个剪接变体（V2 a和V2 b），这两个剪接变体首先在肾脏中被鉴定。可变剪接的V2 b转录物的开放阅读框编码截短的受体，显示与典型V2 a受体相同的氨基酸序列，直到第6个跨膜区段，但相对于V2 a受体显示与相应的第7个跨膜区段和C-末端结构域不同的序列。在这里，我们证明了出生后的表达V2 a和V2 b的变体在大鼠小脑。最重要的是，我们通过原位杂交和免疫细胞化学表明，这两个V2剪接变异体优先在浦肯野细胞中表达，从出生后的早期到晚期的发展。此外，这两种变体在成神经细胞外颗粒细胞和Bergmann纤维中瞬时表达。这些结果表明，这两种剪接变体的细胞分布发育调节，并建议V2受体的瞬时表达参与AVP小脑细胞分化的机制。最后，转染的CHO-K1表达与小脑中发现的相似量的两种V2剪接变体，显示V2 a受体的表面表达显著降低，表明V2剪接变体的差异表达调节小脑中的加压素信号传导。
The V2 vasopressin receptor gene contains an alternative splice site in exon-3, which leads to the generation of two splice variants (V2a and V2b) first identified in the kidney. The open reading frame of the alternatively spliced V2b transcripten codes a truncated receptor, showing the same amino acid sequence as the canonical V2a receptor up to the 6th transmembrane segment, but displaying a distinct sequence to the corresponding 7th transmembrane segment and C-terminal domain relative to the V2a receptor. Here, we demonstrate the postnatal expression of V2a and V2b variants in the rat cerebellum. Most importantly, we showed by in situ hybridization and immunocytochemistry that both V2 splice variants were preferentially expressed in Purkinje cells, from early to late postnatal development. In addition, both variants were transiently expressed in the neuroblastic external granule cells and Bergmann fibers. These results indicate that the cellular distributions of both splice variants are developmentally regulated, and suggest that the transient expression of the V2 receptor is involved in the mechanisms of cerebellar cytodifferentiation by AVP. Finally, transfected CHO-K1 .expressing similar amounts of both V2 splice variants, as that found in the cerebellum, showed a significant reduction in the surface expression of V2a receptors, suggesting that the differential expression of the V2 splice variants regulate the vasopressin signaling in the cerebellum.
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DOI：
10.1073/pnas.88.4.1494
发表时间：
1991-02-01
影响因子：
11.1
作者：
DEWIED, D;ELANDS, J;KOVACS, G
通讯作者：
KOVACS, G
影响因子：
2.5
作者：
ENGELMANN, M;BURES, J;LANDGRAF, R
通讯作者：
LANDGRAF, R
影响因子：
16.2
作者：
Bielsky, IF;Hu, SB;Young, LJ
通讯作者：
Young, LJ
影响因子：
19.6
作者：
Gonzalez, CB;Figueroa, CD;Menzel, D
通讯作者：
Menzel, D
影响因子：
2.9
作者：
CAFFE, AR;VANLEEUWEN, FW;VANDERGUGTEN, J
通讯作者：
VANDERGUGTEN, J