Short-Term Dietary Intervention with Whole Oats Protects from Antibiotic-Induced Dysbiosis.
Short-Term Dietary Intervention with Whole Oats Protects from Antibiotic-Induced Dysbiosis.
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DOI：
10.1128/spectrum.02376-23
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发表时间：
2023-08-17
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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已知抗生素诱导的肠道微生物组生态失调（AID）受宿主膳食组成的影响。然而，饮食如何以及何时调节肠道生态失调仍然缺乏特征。因此，在这里，我们利用多组学方法来表征补充燕麦的饮食，燕麦是微生物可获得的碳水化合物的丰富来源，或葡萄糖如何影响阿莫西林诱导的肠道微生物组结构和转录活性的变化。我们证明，在阿莫西林挑战期间，燕麦给药比总是燕麦或恢复燕麦饮食组提供更大的保护免受艾滋病。特别是，在抗生素暴露时提供燕麦的组诱导了对AID的最大保护，而其他燕麦饮食在阿莫西林挑战后效果更好。与葡萄糖饮食相比，燕麦饮食同样减少了阿莫西林驱动的厚壁菌门的消除。在功能上，饲喂葡萄糖的肠道群落是碳水化合物饥饿的，有利于呼吸代谢和随后的代谢应激管理，而燕麦饲喂的群落改变了它们的转录组学特征，强调抗生素应激管理。当评估以下两种常见的肠道细菌的转录活性时，举例说明了代谢趋势：嗜粘蛋白阿克曼氏菌和多形拟杆菌。这些发现表明，虽然宿主饮食在塑造抗生素如何影响肠道微生物组组成和功能方面很重要，但饮食时机可能在基于饮食干预的治疗中发挥更大的作用。重要性我们利用多组学方法来证明，饮食中补充燕麦，一个丰富的来源微生物可获得的碳水化合物，能够提供保护，防止寄生虫诱导的生态失调（AID）。我们的研究结果证实，宿主饮食不仅在塑造抗生素对肠道微生物组组成和功能的影响方面很重要，而且这些饮食的时机可能在管理艾滋病方面发挥更大的作用。这项工作提供了一个微妙的角度对艾滋病的饮食干预，并可能在常规抗生素治疗预防艾滋病的信息。
Antibiotic-induced gut microbiome dysbiosis (AID) is known to be influenced by host dietary composition. However, how and when diet modulates gut dysbiosis remains poorly characterized. Thus, here, we utilize a multi-omics approach to characterize how a diet supplemented with oats, a rich source of microbiota-accessible carbohydrates, or dextrose impacts amoxicillin-induced changes to gut microbiome structure and transcriptional activity. We demonstrate that oat administration during amoxicillin challenge provides greater protection from AID than the always oats or recovery oats diet groups. In particular, the group in which oats were provided at the time of antibiotic exposure induced the greatest protection against AID while the other oat diets saw greater effects after amoxicillin challenge. The oat diets likewise reduced amoxicillin-driven elimination of Firmicutes compared to the dextrose diet. Functionally, gut communities fed dextrose were carbohydrate starved and favored respiratory metabolism and consequent metabolic stress management while oat-fed communities shifted their transcriptomic profile and emphasized antibiotic stress management. The metabolic trends were exemplified when assessing transcriptional activity of the following two common gut commensal bacteria: Akkermansia muciniphila and Bacteroides thetaiotaomicron. These findings demonstrate that while host diet is important in shaping how antibiotics effect the gut microbiome composition and function, diet timing may play an even greater role in dietary intervention-based therapeutics. IMPORTANCE We utilize a multi-omics approach to demonstrate that diets supplemented with oats, a rich source of microbiota-accessible carbohydrates, are able to confer protection against antibiotic-induced dysbiosis (AID). Our findings affirm that not only is host diet important in shaping antibiotics effects on gut microbiome composition and function but also that the timing of these diets may play an even greater role in managing AID. This work provides a nuanced perspective on dietary intervention against AID and may be informative on preventing AID during routine antibiotic treatment.
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影响因子：
7.7
作者：
Beghini F;McIver LJ;Blanco-Míguez A;Dubois L;Asnicar F;Maharjan S;Mailyan A;Manghi P;Scholz M;Thomas AM;Valles-Colomer M;Weingart G;Zhang Y;Zolfo M;Huttenhower C;Franzosa EA;Segata N
通讯作者：
Segata N
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
14.9
作者：
Caspi R;Altman T;Dale JM;Dreher K;Fulcher CA;Gilham F;Kaipa P;Karthikeyan AS;Kothari A;Krummenacker M;Latendresse M;Mueller LA;Paley S;Popescu L;Pujar A;Shearer AG;Zhang P;Karp PD
通讯作者：
Karp PD
影响因子：
6.4
作者：
Bald D;Villellas C;Lu P;Koul A
通讯作者：
Koul A
DOI：
10.1038/nrg3182
发表时间：
2012-03-13
期刊：
Nature reviews. Genetics
影响因子：
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