PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar
PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar
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PtrMYB57 有助于杨树花青素和原花青素生物合成的负调控
DOI：
10.1007/s00299-017-2151-y
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发表时间：
2017-05
影响因子：
6.2
通讯作者：
Luo Keming
中科院分区：
文献类型：
--
作者：
Wan Shuzhen;Li Chaofeng;Ma Xiaodong;Luo Keming
Key messageA novel R2R3 MYB transcription factorPtrMYB57interacted with bHLH131 and PtrTTG1 to form the MBW complex and negatively regulated the biosynthesis of both anthocyanins and PAs in poplar.AbstractR2R3-MYB transcription factors (TFs) are important regulators of secondary metabolite biosynthesis in woody species. A series of R2R3-MYB TFs involved in anthocyanin and proanthocyanidin (PA) biosynthesis have been identified in poplar. In this study, we report the identification and characterization of a subgroup 4 MYB member PtrMYB57, which contains a repressor domain (LxLxL) at the C-terminal end.PtrMYB57encodes an R2R3 MYB protein localized in the nucleus and is predominantly expressed in mature leaves. Transgenic poplar overexpressingPtrMYB57showed a reduction in anthocyanin and PA accumulation compared to wild-type plants. By contrast, a high anthocyanin and PA phenotype was observed inPtrmyb57mutants generated by the CRISPR/Cas9 system. Furthermore, transient expression assays revealed that PtrMYB57 interacted with bHLH131 (bHLH) and PtrTTG1 (WDR) to form the MBW complex and bound to the flavonoid gene promoters, leading to inhibition of these promoters. Taken together, our results suggest that PtrMYB57 plays a negative role in the regulation of anthocyanin and PA biosynthesis in poplar.
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影响因子：
5.6
作者：
Albert NW
通讯作者：
Albert NW
DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
--
作者：
F. Xu;R. Cai;S. Cheng;H. Du;Yan Wang
通讯作者：
F. Xu;R. Cai;S. Cheng;H. Du;Yan Wang
影响因子：
7.2
作者：
Walker, Amanda R.;Lee, Elizabeth;Robinson, Simon P.
通讯作者：
Robinson, Simon P.
影响因子：
6.9
作者：
Colquhoun TA;Kim JY;Wedde AE;Levin LA;Schmitt KC;Schuurink RC;Clark DG
通讯作者：
Clark DG