Probiotics Function as Immunomodulators in the Intestine in C57Bl/6 Male Mice Exposed to Inhaled Diesel Exhaust Particles on a High-Fat Diet.
Probiotics Function as Immunomodulators in the Intestine in C57Bl/6 Male Mice Exposed to Inhaled Diesel Exhaust Particles on a High-Fat Diet.
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DOI：
10.3390/cells11091445
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发表时间：
2022-04-25
期刊：
影响因子：
6
通讯作者：
Lund, Amie K.
中科院分区：
文献类型：
--
作者：
Phillippi, Danielle T.;Daniel, Sarah;Nguyen, Kayla N.;Penaredondo, Bea Angella;Lund, Amie K.
关键词：
流行病学研究揭示了空气污染暴露与胃肠道 (GI) 疾病之间的相关性，但很少有研究调查吸入颗粒物与高脂肪 (HF) 饮食相结合对肠道完整性的作用。此外，目前关于益生菌减轻肠道空气污染物反应的信息有限。因此，我们研究了这样的假设：接触吸入柴油机尾气颗粒 (DEP) 和高频饮食会改变肠道完整性和炎症，而益生菌可以减轻肠道完整性和炎症。采用 HF 饮食（45% kcal 脂肪）的 4-6 周龄雄性 C57Bl/6 小鼠被随机分配通过口咽抽吸暴露于悬浮在 35 µL 0.9% 无菌盐水或无菌盐水 (CON) 中的 35 µg DEP，每周两次，持续 4 周。在整个研究期间，一组小鼠每天接受 0.3 克 Winclove Ecologic® 屏障益生菌 (PRO) 的饮用水治疗。我们的结果表明，通过 AB/PAS 染色确定，DEP 暴露 ± 益生菌导致杯状细胞和粘蛋白 (MUC)-2 表达增加。免疫荧光定量和/或 RT-qPCR 显示，与 CON 相比，DEP 暴露增加了claudin-3、occludin、闭塞带 (ZO)-1、基质金属蛋白酶 (MMP)-9 和 Toll 样受体 (TLR)-4，并降低了肿瘤坏死因子 (TNF)-α 和白细胞介素 (IL)-10 的表达。与 CON + PRO 相比，DEP 暴露 + 益生菌会增加小肠中claudin-3、occludin、ZO-1、TNF-α 和 IL-10 的表达，并降低 MMP-9 和 TLR-4。总的来说，这些结果表明 DEP 暴露与 HF 饮食一起改变肠道完整性和炎症。事实证明，益生菌对于了解微生物组在接触吸入 DEP 后保护和改变肠道炎症反应中的作用至关重要。
Epidemiological studies reveal a correlation between air pollution exposure and gastrointestinal (GI) diseases, yet few studies have investigated the role of inhaled particulate matter on intestinal integrity in conjunction with a high-fat (HF) diet. Additionally, there is currently limited information on probiotics in mitigating air-pollutant responses in the intestines. Thus, we investigated the hypothesis that exposure to inhaled diesel exhaust particles (DEP) and a HF diet can alter intestinal integrity and inflammation, which can be attenuated with probiotics. 4–6-w-old male C57Bl/6 mice on a HF diet (45% kcal fat) were randomly assigned to be exposed via oropharyngeal aspiration to 35 µg of DEP suspended in 35 µL of 0.9% sterile saline or sterile saline (CON) only twice a week for 4 w. A subset of mice was treated with 0.3 g/day of Winclove Ecologic® barrier probiotics (PRO) in drinking water throughout the duration of the study. Our results show that DEP exposure ± probiotics resulted in increased goblet cells and mucin (MUC)-2 expression, as determined by AB/PAS staining. Immunofluorescent quantification and/or RT-qPCR showed that DEP exposure increases claudin-3, occludin, zona occludens (ZO)-1, matrix metalloproteinase (MMP)-9, and toll-like receptor (TLR)-4, and decreases tumor necrosis factor (TNF)-α and interleukin (IL)-10 expression compared to CON. DEP exposure + probiotics increases expression of claudin-3, occludin, ZO-1, TNF-α, and IL-10 and decreases MMP-9 and TLR-4 compared to CON + PRO in the small intestine. Collectively, these results show that DEP exposure alters intestinal integrity and inflammation in conjunction with a HF diet. Probiotics proved fundamental in understanding the role of the microbiome in protecting and altering inflammatory responses in the intestines following exposure to inhaled DEP.
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影响因子：
--
作者：
Fulda S;Gorman AM;Hori O;Samali A
通讯作者：
Samali A
DOI：
10.1038/nri.2016.88
发表时间：
2016-10
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Johansson ME;Hansson GC
通讯作者：
Hansson GC
影响因子：
4.6
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通讯作者：
Hasnain SZ
影响因子：
10
作者：
Daniel S;Phillippi D;Schneider LJ;Nguyen KN;Mirpuri J;Lund AK
通讯作者：
Lund AK
影响因子：
4.9
作者：
Ananthakrishnan, Ashwin N.;McGinley, Emily L.;Saeian, Kia
通讯作者：
Saeian, Kia