Role of Methoprene-tolerant in the regulation of oogenesis in Dipetalogaster maxima.
Role of Methoprene-tolerant in the regulation of oogenesis in Dipetalogaster maxima.
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DOI：
10.1038/s41598-022-18384-5
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发表时间：
2022-08-20
影响因子：
4.6
通讯作者：
Canavoso, Lilian E.
中科院分区：
文献类型：
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作者：
Ramos, Fabian O.;Nouzova, Marcela;Fruttero, Leonardo L.;Leyria, Jimena;Ligabue-Braun, Rodrigo;Noriega, Fernando G.;Canavoso, Lilian E.
保幼激素（JH）信号通过其受体甲氧普仑耐受（Met）控制昆虫的变态和繁殖。Met属于转录因子的超家族，其包含基本的HLH环HLH（HLH）和Per Arnt Sim（PAS）结构域。自1986年发现以来，Met已在几种昆虫物种中得到表征。然而，尽管重要性作为载体的南美锥虫病，我们的知识的作用，甲硫氨酸在JH信号在锥蝽亚科是有限的。本研究克隆并测序了巨型扇瓣腹菌Met基因的转录本DmaxMet。分子建模用于构建Met的结构并鉴定JH结合位点。为了进一步了解JH受体在卵子发生过程中的作用，在JH的两个主要靶器官，脂肪体和卵巢中评估转录水平。使用Met RNAi的功能研究揭示了卵黄蛋白原（Vg）和脂转运蛋白（Lp）及其受体的转录物的显着减少。脂肪体中Lp、Vg蛋白含量及血淋巴中Vg含量均降低，卵巢发育受损。总的来说，这些研究提供了关于JH信号在锥蝽亚科卵子发生中的作用的额外分子见解;因此与恰加斯病的流行病学有关。
Juvenile hormone (JH) signalling, via its receptor Methoprene-tolerant (Met), controls metamorphosis and reproduction in insects. Met belongs to a superfamily of transcription factors containing the basic Helix Loop Helix (bHLH) and Per Arnt Sim (PAS) domains. Since its discovery in 1986, Met has been characterized in several insect species. However, in spite of the importance as vectors of Chagas disease, our knowledge on the role of Met in JH signalling in Triatominae is limited. In this study, we cloned and sequenced the Dipetalogaster maxima Met transcript (DmaxMet). Molecular modelling was used to build the structure of Met and identify the JH binding site. To further understand the role of the JH receptor during oogenesis, transcript levels were evaluated in two main target organs of JH, fat body and ovary. Functional studies using Met RNAi revealed significant decreases of transcripts for vitellogenin (Vg) and lipophorin (Lp), as well as their receptors. Lp and Vg protein amounts in fat body, as well as Vg in hemolymph were also decreased, and ovarian development was impaired. Overall, these studies provide additional molecular insights on the roles of JH signalling in oogenesis in Triatominae; and therefore are relevant for the epidemiology of Chagas´ disease.
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影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
DOI：
10.1073/pnas.1116123109
发表时间：
2011-12-27
影响因子：
11.1
作者：
Charles, Jean-Philippe;Iwema, Thomas;Jindra, Marek
通讯作者：
Jindra, Marek
影响因子：
2.2
作者：
Leyria, Jimena;Fruttero, Leonardo L.;Canavoso, Lilian E.
通讯作者：
Canavoso, Lilian E.
影响因子：
3.8
作者：
Aguirre, Silvina A.;Fruttero, Leonardo L.;Canavoso, Lilian E.
通讯作者：
Canavoso, Lilian E.
德国蟑螂亲脂蛋白受体以及脂蛋白受体的进化关系的结构和RNAi表征。
影响因子：
--
作者：
Ciudad, Laura;Belles, Xavier;Piulachs, Maria-Dolors
通讯作者：
Piulachs, Maria-Dolors