Structural and RNAi characterization of the German cockroach lipophorin receptor, and the evolutionary relationships of lipoprotein receptors.
Structural and RNAi characterization of the German cockroach lipophorin receptor, and the evolutionary relationships of lipoprotein receptors.
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德国蟑螂亲脂蛋白受体以及脂蛋白受体的进化关系的结构和RNAi表征。
DOI：
10.1186/1471-2199-8-53
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发表时间：
2007-06-22
影响因子：
--
通讯作者：
Piulachs, Maria-Dolors
中科院分区：
文献类型：
--
作者：
Ciudad, Laura;Belles, Xavier;Piulachs, Maria-Dolors
载脂蛋白受体（Lipophorin receptor，LpRs）在许多昆虫中都有发现，但功能性研究仅在蝗虫和蚊子中有报道。本工作的目的是表征的LpR的蟑螂德国小蠊，不仅在分子上，而且功能上使用RNAi技术，并放置LpRs在脂蛋白受体之间的遗传背景。我们从B克隆了一个假定的LpR。germanica（BgLpR）的基因。从脂肪体和卵巢中获得了两种BgLpR亚型，它们通过24个氨基酸的插入/缺失而彼此不同。脂蛋白受体的遗传学分析表明，BgLpR与其他注释为LpR的序列聚在一起，作为脊椎动物低密度脂蛋白受体（LDLR）+脂蛋白受体相关蛋白8（LPR 8）+卵黄蛋白原受体（VgR）+极低密度脂蛋白受体（VLDLR）的姐妹群。两种BgLpR亚型在不同的成年雌性组织（脂肪体、卵巢、脑、中肠、肌肉）和胚胎中表达。在卵巢和脂肪体中，这两种亚型在第一个促性腺周期中表达相似。脂肪体中的mRNA水平与卵黄发生平行增加，而卵巢中的mRNA水平降低。BgLpR蛋白水平的增加平行于两个器官中的卵黄发生。用保幼激素处理增加BgLpR蛋白。RNAi实验表明，与对照相比，具有较低BgLpR表达的雌性在生长的卵母细胞中具有较少的载脂蛋白。BgLpR的两种亚型在结构上与其他LpR相似。系统发育分析表明，LpRs与脊椎动物LDLR + LPR 8 + VgR + VLDLR组成的类群，从一个共同的祖先分化出来，并平行地多样化。比较mRNA和蛋白质，脂肪体和卵巢中的不同表达模式表明调节BgLpR表达的相应机制不同。JH III的实验表明，这种激素在转录后调节BgLpR的表达。RNAi实验表明BgLpR是一种功能性的载脂蛋白受体。
Lipophorin receptors (LpRs) have been described in a number of insects, but functional studies have been reported only in locusts and mosquitoes. The aim of the present work was to characterize the LpR of the cockroach Blattella germanica, not only molecularly but also functionally using RNAi techniques, and to place LpRs in a phylogenetical context among lipoprotein receptors. We cloned a putative LpR from B. germanica (BgLpR) using RT-PCR methods. Two isoforms of BgLpR that differ from each other by an insertion/deletion of 24 amino acids were obtained from the fat body and the ovary. A phylogenetical analysis of lipoprotein receptors showed that BgLpR grouped with other sequences annotated as LpR in a cluster placed as a sister group of vertebrate low density lipoprotein receptors (LDLR) + lipoprotein receptor-related proteins 8 (LPR8) + vitellogenin receptors (VgR) + very low density lipoprotein receptors (VLDLR). The two BgLpR isoforms are expressed in different adult female tissues (fat body, ovary, brain, midgut, muscle) and in embryos. In ovaries and fat body, the two isoforms are similarly expressed during the first gonadotrophic cycle. mRNA levels in the fat body increase in parallel to vitellogenesis, whereas they decrease in the ovaries. BgLpR protein levels increase in parallel to vitellogenesis in both organs. Treatment with juvenile hormone increases BgLpR protein. RNAi experiments show that females with lower BgLpR expression have less lipophorin in the growing oocytes with respect to controls. The two isoforms of BgLpR are structurally similar to other LpRs. Phylogenetical analyses show that LpRs and the group formed by vertebrate LDLR + LPR8 + VgR + VLDLR, diverged from a common ancestor and diversified in parallel. The different expression patterns in the fat body and the ovary, comparing mRNA and protein, indicate that the corresponding mechanisms regulating BgLpR expression are different. Experiments with JH III suggest that such a hormone regulates the expression of BgLpR posttranscriptionally. RNAi experiments indicate that BgLpR is a functional lipophorin receptor.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
2.2
作者：
KUNKEL, JG;PAN, ML
通讯作者：
PAN, ML
影响因子：
3.8
作者：
Cheon, HM;Seo, SJ;Raikhel, AS
通讯作者：
Raikhel, AS
影响因子：
3.5
作者：
Monné, M;von Heijne, G
通讯作者：
von Heijne, G
DOI：
10.1073/pnas.2234416100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Zhu, JS;Chen, L;Raikhel, AS
通讯作者：
Raikhel, AS