From lateral flow devices to a novel nano-color microfluidic assay.
From lateral flow devices to a novel nano-color microfluidic assay.
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DOI：
10.3390/s90806084
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Schalkhammer T
中科院分区：
文献类型：
--
作者：
Assadollahi S;Reininger C;Palkovits R;Pointl P;Schalkhammer T
结合新的制造技术改进传统诊断侧流分析的性能是诊断公司研发计划的主要目标。考虑到侧流诊断测试套件的组件;创新可以包括标签、材料和设备设计的修改。近年来，金属纳米粒子的共振增强吸收（REA）在生物传感中表现出优异的适用性，用于检测各种生物分子之间的相互作用。在一种新的方法中，我们现在已经将REA测定集成在诊断微流体设置中，从而解决了先前现有的REA测定中固有的长孵育时间的瓶颈，并同时集成了用于诊断制造的自动化制造技术。由于基于辊涂的技术和耐化学性，我们使用PET-共聚酯作为基材，并使用CO2激光烧蚀系统作为传统微铣削的快速、高精度和非接触式替代方案。可以在三分钟内检测生物结合-在REA流体装置内作为彩色文本读数对眼睛可见。如果需要，两分钟的原位银增强能够额外增强共振颜色。
Improving the performance of traditional diagnostic lateral flow assays combined with new manufacturing technologies is a primary goal in the research and development plans of diagnostic companies. Taking into consideration the components of lateral flow diagnostic test kits; innovation can include modification of labels, materials and device design. In recent years, Resonance-Enhanced Absorption (REA) of metal nano-particles has shown excellent applicability in bio-sensing for the detection of a variety of bio-molecular binding interactions. In a novel approach, we have now integrated REA-assays in a diagnostic microfluidic setup thus resolving the bottleneck of long incubation times inherent in previously existing REA-assays and simultaneously integrated automated fabrication techniques for diagnostics manufacture. Due to the roller-coating based technology and chemical resistance, we used PET-co-polyester as a substrate and a CO2 laser ablation system as a fast, highly precise and contactless alternative to classical micro-milling. It was possible to detect biological binding within three minutes – visible to the eye as colored text readout within the REA-fluidic device. A two-minute in-situ silver enhancement was able to enhance the resonant color additionally, if required.
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影响因子：
3.3
作者：
Ayong, LS;Tume, CB;Ngu, JL
通讯作者：
Ngu, JL
影响因子：
9.4
作者：
Mettler, M;Grimm, F;Deplazes, P
通讯作者：
Deplazes, P
影响因子：
56.9
作者：
Edwards, Jennifer K.;Solsona, Benjamin;Hutchings, Graham J.
通讯作者：
Hutchings, Graham J.
具有受控壳厚度的钯包金纳米颗粒用作表面增强拉曼散射基底
影响因子：
3.7
作者：
Hu, Jia-Wen;Li, Jian-Feng;Tian, Zhong-Qun
通讯作者：
Tian, Zhong-Qun
DOI：
10.1007/bf00592566
发表时间：
1981-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
DANSCHER, G
通讯作者：
DANSCHER, G