A gene-rich linkage map in the dioecious species Actinidia chinensis (kiwifruit) reveals putative X/Y sex-determining chromosomes.
A gene-rich linkage map in the dioecious species Actinidia chinensis (kiwifruit) reveals putative X/Y sex-determining chromosomes.
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雌雄异株猕猴桃（猕猴桃）中富含基因的连锁图谱揭示了假定的 X/Y 性别决定染色体。
DOI：
10.1186/1471-2164-10-102
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发表时间：
2009-03-10
期刊：
影响因子：
4.4
通讯作者：
McNeilage, Mark A.
中科院分区：
文献类型：
--
作者：
Fraser, Lena G.;Tsang, Gianna K.;Datson, Paul M.;De Silva, H. Nihal;Harvey, Catherine F.;Gill, Geoffrey P.;Crowhurst, Ross N.;McNeilage, Mark A.
猕猴桃属（猕猴桃）由木质，攀缘藤蔓组成，原产于中国，最近才作为商业作物繁殖。所有被描述的物种都是雌雄异株，但性别决定的遗传机制尚不清楚，正如构成独特果实的许多特征的遗传基础一样。然而，它在新西兰经济中是一种重要的作物，而一个传统的育种计划将极大地受益于对雌性和雄性父母携带的性状等位基因的了解。标记辅助选择（MAS）技术在苗木群体中的应用，也有助于准确、高效地培育出面向市场的新品种。利用644个微卫星标记，构建了二倍体中华古树的富基因雌性、雄性和共识连锁图谱。该图谱由29个连锁组组成，对应于单倍体数n = 29。我们发现，性别连锁序列特征扩增区（SCAR）标记和“花性”表型一致地映射到亚端粒区域的单个连锁组，在不一致的标记顺序部分中。该区域还包含表达基因的标记，其中一些功能未知。通过等位基因分布和标记顺序稳定性评估的重组，在其余连锁组中，与其他连锁组一致。该连锁组中其他基因的完全信息标记确定了女性图谱中的比较连锁组，其中决定标记顺序的重组比率与常染色体相似。建立了中国古猿单倍体基因组29个连锁群的遗传连锁图谱，揭示了中国古猿性别决定位点的位置和范围。由于猕猴桃属所有物种都是雌雄异株，我们认为其他猕猴桃属物种的性别决定位点将与我们在地图中定义的区域相似。由于非重组区域的范围有限，我们的结果支持常染色体亚端粒区域处于发展性染色体特征的早期阶段的建议。这些图谱为猕猴桃属植物的遗传分析和育种提供了参考。
The genus Actinidia (kiwifruit) consists of woody, scrambling vines, native to China, and only recently propagated as a commercial crop. All species described are dioecious, but the genetic mechanism for sex-determination is unknown, as is the genetic basis for many of the cluster of characteristics making up the unique fruit. It is, however, an important crop in the New Zealand economy, and a classical breeding program would benefit greatly by knowledge of the trait alleles carried by both female and male parents. The application of marker assisted selection (MAS) in seedling populations would also aid the accurate and efficient development of novel fruit types for the market. Gene-rich female, male and consensus linkage maps of the diploid species A. chinensis have been constructed with 644 microsatellite markers. The maps consist of twenty-nine linkage groups corresponding to the haploid number n = 29. We found that sex-linked sequence characterized amplified region (SCAR) markers and the 'Flower-sex' phenotype consistently mapped to a single linkage group, in a subtelomeric region, in a section of inconsistent marker order. The region also contained markers of expressed genes, some of unknown function. Recombination, assessed by allelic distribution and marker order stability, was, in the remainder of the linkage group, in accordance with other linkage groups. Fully informative markers to other genes in this linkage group identified the comparative linkage group in the female map, where recombination ratios determining marker order were similar to the autosomes. We have created genetic linkage maps that define the 29 linkage groups of the haploid genome, and have revealed the position and extent of the sex-determining locus in A. chinensis. As all Actinidia species are dioecious, we suggest that the sex-determining loci of other Actinidia species will be similar to that region defined in our maps. As the extent of the non-recombining region is limited, our result supports the suggestion that the subtelomeric region of an autosome is in the early stages of developing the characteristics of a sex chromosome. The maps provide a reference of genetic information in Actinidia for use in genetic analysis and breeding programs.
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影响因子：
5.4
作者：
Fraser, LG;Harvey, CF;De Silva, HN
通讯作者：
De Silva, HN
影响因子：
4.9
作者：
HUGHES, CR;QUELLER, DC
通讯作者：
QUELLER, DC
影响因子：
3.3
作者：
Kim, Jung Sun;Chung, Tae Young;Park, Beom-Seok
通讯作者：
Park, Beom-Seok
影响因子：
4.4
作者：
Crowhurst RN;Gleave AP;MacRae EA;Ampomah-Dwamena C;Atkinson RG;Beuning LL;Bulley SM;Chagne D;Marsh KB;Matich AJ;Montefiori M;Newcomb RD;Schaffer RJ;Usadel B;Allan AC;Boldingh HL;Bowen JH;Davy MW;Eckloff R;Ferguson AR;Fraser LG;Gera E;Hellens RP;Janssen BJ;Klages K;Lo KR;MacDiarmid RM;Nain B;McNeilage MA;Rassam M;Richardson AC;Rikkerink EH;Ross GS;Schröder R;Snowden KC;Souleyre EJ;Templeton MD;Walton EF;Wang D;Wang MY;Wang YY;Wood M;Wu R;Yauk YK;Laing WA
通讯作者：
Laing WA
影响因子：
5.4
作者：
Butcher, PA;Williams, ER;Moran, GF
通讯作者：
Moran, GF